Torres Reinaldo, Hurtado Claudio, Pérez-Macchi Sandra, Bittencourt Pedro, Freschi Carla, de Mello Victoria Valente Califre, Machado Rosangela Zacarias, André Marcos Rogério, Müller Ananda
Instituto de Ciencias Clinicas Veterinarias, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, Chile.
Departamento de Patologia Clinica Veterinaria, Facultad de Ciencias Veterinarias, Universidad Nacional de Asunción, San Lorenzo 1114, Paraguay.
Pathogens. 2021 Jun 7;10(6):714. doi: 10.3390/pathogens10060714.
This study aimed to serologically and molecularly survey and in thoroughbred horses from racecourses in Chile. Additionally, the genetic diversity of the positive samples was assessed. A total of 286 thoroughbred horses from the Santiago and Valparaíso racecourses had their serum samples submitted to an ELISA for and , and 457 samples (from the Santiago, Valparaíso, and Concepción racecourses) were tested with nested PCRs for the 48 KDa rhoptry protein (RAP-1) and 18S rRNA genes. Selected RAP-1 and 18S positive products were sequenced to perform phylogenetic and haplotype analyses. An overall seroprevalence of 35.6% was observed for these Chilean racecourses: 23.7% for 8.4% for , and 3.5% for both agents. Overall, a 53.6% occurrence by nPCR was detected for the three Chilean racecourses: 44.2% for , 5.4% for , and 3.9% for both agents. Phylogenetic analysis of and showed genetic proximity with sequences previously detected in other countries. Haplotype analysis revealed a low diversity among the Chilean sequences, which may have originated from those reported in Brazil, Israel, or Cuba. and were detected for the first time in Chilean thoroughbred horses.
本研究旨在对智利赛马场的纯种马进行血清学和分子学调查，并评估阳性样本的遗传多样性。来自圣地亚哥和瓦尔帕莱索赛马场的总共286匹纯种马的血清样本被送去进行针对[具体病原体1]和[具体病原体2]的酶联免疫吸附测定（ELISA），并且457个样本（来自圣地亚哥、瓦尔帕莱索和康塞普西翁赛马场）用巢式聚合酶链反应（nested PCR）检测48千道尔顿棒状体蛋白（RAP - 1）和18S核糖体RNA（rRNA）基因。对选定的RAP - 1和18S阳性产物进行测序以进行系统发育和单倍型分析。在这些智利赛马场观察到总体血清阳性率为35.6%：[具体病原体1]为23.7%，[具体病原体2]为8.4%，两种病原体均为3.5%。总体而言，在这三个智利赛马场通过巢式聚合酶链反应检测到的发生率为53.6%：[具体病原体1]为44.2%，[具体病原体2]为5.4%，两种病原体均为3.9%。对[具体病原体1]和[具体病原体2]的系统发育分析表明与先前在其他国家检测到的序列具有遗传相似性。单倍型分析显示智利序列中的多样性较低，这可能源于巴西、以色列或古巴报道的那些序列。[具体病原体1]和[具体病原体2]首次在智利纯种马中被检测到。
