Durrens P, Green P M, Arst H N, Scazzocchio C
Mol Gen Genet. 1986 Jun;203(3):544-9. doi: 10.1007/BF00422084.
The analysis of four transformants for the proline catabolism (prn) gene cluster of Aspergillus nidulans is reported. Using a combination of traditional genetic methodology and Southern hybridisation we have shown that in two cases multiple copies of the transforming plasmid have been integrated into linkage groups other than VII, which contains the prn cluster. In the other two cases integration of the plasmid has probably occurred homologously. The phenotype of these transformants is broadly consistent with increased copy number resulting in increased expression. Genetic manipulation of these transformants using the sexual or parasexual cycles has shown that recombination events during and possibly also subsequent to integration of the transforming DNA can generate new mutational lesions, in particular, deletions.
本文报道了对构巢曲霉脯氨酸分解代谢（prn）基因簇的四个转化体的分析。通过结合传统遗传方法和Southern杂交，我们发现，在两个案例中，转化质粒的多个拷贝已整合到除包含prn基因簇的VII连锁群以外的其他连锁群中。在另外两个案例中，质粒的整合可能是同源发生的。这些转化体的表型与拷贝数增加导致表达增加大致相符。利用有性或准性生殖周期对这些转化体进行遗传操作表明，在转化DNA整合期间以及可能在整合之后发生的重组事件可产生新的突变损伤，特别是缺失。
