用粗糙脉孢菌的乳清酸核苷-5'-磷酸脱羧酶基因转化构巢曲霉。

Transformation of Aspergillus nidulans by the orotidine-5'-phosphate decarboxylase gene of Neurospora crassa.

作者信息

Ballance D J, Buxton F P, Turner G

出版信息

Biochem Biophys Res Commun. 1983 Apr 15;112(1):284-9. doi: 10.1016/0006-291x(83)91828-4.

PMID:6220717

Abstract

Relief of an auxotrophic requirement for uridine in Aspergillus nidulans strain G191 has been achieved by transformation with a segment of Neurospora crassa DNA containing the corresponding gene coding for orotidine-5'-phosphate decarboxylase. The mitotic stability of such transformants suggests that the DNA has integrated into the genome. Southern hybridisation analysis of DNA isolated from transformants revealed the presence of pBR322 sequences which have integrated into the host genome along with the N. crassa DNA.

摘要

通过用含有编码乳清苷 -5'-磷酸脱羧酶的相应基因的粗糙脉孢菌DNA片段进行转化，已实现了构巢曲霉G191菌株对尿苷营养缺陷型需求的缓解。此类转化体的有丝分裂稳定性表明该DNA已整合到基因组中。对从转化体中分离的DNA进行Southern杂交分析，揭示了pBR322序列的存在，这些序列已与粗糙脉孢菌DNA一起整合到宿主基因组中。