College of Materials Science and Engineering, Nanjing Tech University, Nanjing 210009, China; Nanjing Haoqi Advanced Materials Co., Ltd., Nanjing 211300, China.
College of Materials Science and Engineering, Nanjing Tech University, Nanjing 210009, China.
Mater Sci Eng C Mater Biol Appl. 2018 Nov 1;92:1-10. doi: 10.1016/j.msec.2018.06.003. Epub 2018 Jun 15.
Vaterite-containing β-dicalcium silicate powder (A-V-CS) was successfully grafting modified by 3-aminopropyltriethoxysilane (APTES) through reflux agitation. The purpose of this work is to fix the amino groups on the vaterite-containing β-dicalcium silicate (V-CS) powder's surface, which makes the powder's surface with positive charge, and further enhanced the combination with those negatively charged bioactive molecules or drugs to improve the performance of biomaterials. XRD and FT-IR analysis indicated that N-H groups were successfully grafted onto the surface of the V-CS sample on the basis of vaterite existence through the process of first grafting then carbonation. Moreover, the best grafting modification that refluxing at 70 °C for 12 h was determined through the quantitative analysis of N-H groups on V-CS powder's surface. The A-V-CS sample showed a better apatite formation ability after it was soaked in stimulated body fluid (SBF), indicating that good apatite mineralization. Moreover, the A-V-CS sample with weak alkali in implanting position could stimulate the attachment, proliferation and improve the activity of MC3T3-E1 cells. Experimental results demonstrated that, A-V-CS was expected to be a new biomaterial as bone repairing substitute.
载负文石相的β-双硅酸钙粉体（A-V-CS）通过回流搅拌成功地用 3-氨丙基三乙氧基硅烷（APTES）进行了接枝改性。本工作的目的是固定载负文石相的β-双硅酸钙（V-CS）粉体表面上的氨基，使粉体表面带正电荷，进一步增强与那些带负电荷的生物活性分子或药物的结合，从而提高生物材料的性能。XRD 和 FT-IR 分析表明，基于文石的存在，通过先接枝后碳化的过程，N-H 基团成功地接枝到 V-CS 样品表面上。此外，通过对 V-CS 粉体表面上 N-H 基团的定量分析，确定了在 70°C 下回流 12 h 的最佳接枝改性条件。A-V-CS 样品在浸泡于模拟体液（SBF）后表现出更好的磷灰石形成能力，表明具有良好的磷灰石矿化。此外，在植入部位呈弱碱性的 A-V-CS 样品能够刺激 MC3T3-E1 细胞的附着、增殖和提高其活性。实验结果表明，A-V-CS 有望成为一种新的骨修复替代材料。
