College of Materials Science and Engineering, Nanjing Tech University, Nanjing 210009, People's Republic of China.
Biomed Mater. 2017 Oct 25;12(6):065007. doi: 10.1088/1748-605X/aa84b8.
A novel vaterite-containing tricalcium silicate (V-CS) was grafted by 3-aminopropyltriethoxysilane (APTES), and the amino groups have been successfully fixed on the vaterite-containing tricalcium silicate powder's surface (after grafting the amino group, V-CS was named A-V-CS). The setting behavior, mechanical properties, porosity, weight loss and anti-washout properties of the tricalcium silicate (CS), V-CS and A-V-CS bone cement were systematically investigated. The in vitro induction of hydroxyapatite (HAp) formation of CS, V-CS and A-V-CS bone cement was confirmed by x-ray diffraction, Fourier-transform infrared spectroscopy and scanning electron microscopy. The cell viability, cell proliferation and cell attachment were investigated to assess the effects of bone cement on MC3T3-E1 cells. Results showed that the setting time of A-V-CS bone cement can meet the requirements of a clinical test, with improved anti-washout properties and an appropriate degradation rate. The pH value of the soaking solution was obviously decreased by surface modification. Besides, the morphology and fluorescence photograph results revealed that the A-V-CS bone cement showed an enhanced biocompatibility effect on the proliferation and attachment of MC3T3-E1 cells. The A-V-CS bone cement was expected to be a potential bone-substitute material.
一种新型的含文石的硅酸三钙（V-CS）通过 3-氨丙基三乙氧基硅烷（APTES）接枝，氨基基团已经成功固定在含文石的硅酸三钙粉末的表面（接枝氨基基团后，V-CS 被命名为 A-V-CS）。系统研究了硅酸三钙（CS）、V-CS 和 A-V-CS 骨水泥的凝固行为、力学性能、孔隙率、失重和抗冲刷性能。通过 X 射线衍射、傅里叶变换红外光谱和扫描电子显微镜证实了 CS、V-CS 和 A-V-CS 骨水泥体外诱导羟基磷灰石（HAp）形成。通过评估骨水泥对 MC3T3-E1 细胞的细胞活力、细胞增殖和细胞附着来研究其效果。结果表明，A-V-CS 骨水泥的凝固时间可以满足临床测试的要求，具有改善的抗冲刷性能和适当的降解率。表面改性明显降低了浸出液的 pH 值。此外，形态和荧光照片结果表明，A-V-CS 骨水泥对 MC3T3-E1 细胞的增殖和附着具有增强的生物相容性作用。A-V-CS 骨水泥有望成为一种有潜力的骨替代材料。
