College of Materials Science and Engineering, Nanjing Tech University, Nanjing 210009, China; Nanjing Haoqi Advanced Materials Co., Ltd., Nanjing 211300, China.
College of Materials Science and Engineering, Nanjing Tech University, Nanjing 210009, China.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:23-29. doi: 10.1016/j.msec.2017.05.025. Epub 2017 May 5.
A novel bioactive vaterite-containing tricalcium silicate bone cement (V5) was successfully synthesized through self hydration and carbonization, by introducing CO into the hydration process of CaSiO (CS). The purpose of this work is to reduce the adverse effect of the hydration products Ca(OH) on the CS bone cement, and improve further the bioactivity of the CS bone cement. XRD and FT-IR analysis indicated that vaterite was successfully formed in tricalcium silicate bone cement. The in vitro bioactivity of V5 was investigated by soaking in simulated body fluid (SBF) for various periods (1, 3, 7days), in addition to setting time, compressive strength and cell behavior. The results showed that the V5 could rapidly induce hydroxyapatite (HA) formation. The bio-effects of V5 on MC3T3-E1 osteoblast-like cells were evaluated by studying cell viability, adhesion and proliferation. The CCK-8 assay shows that cell viability on the resulting V5 is improved obviously after through hydration and carbonization. The V5 cement enhanced the higher expression of cell attachment, proliferation and differentiation as compared to V50 cement. SEM results present significant improvement on the cell adhesion and proliferation for cells cultured on the V5. Experimental results demonstrated that, CS bone cement of containing vaterite has more excellent bioactivity and medium setting time too, suggesting their potential applications in areas such as MC3T3-E1 cell stimulation and bone tissue engineering.
一种新型的含碳酸化相的生物活性硅酸三钙骨水泥（V5）通过将 CO2 引入 CaSiO3 的水化过程中，成功地通过自水合和碳化作用合成。本工作的目的是降低水化产物 Ca(OH)2 对 CS 骨水泥的不利影响，并进一步提高 CS 骨水泥的生物活性。XRD 和 FT-IR 分析表明，在硅酸三钙骨水泥中成功地形成了方解石。通过将 V5 在模拟体液（SBF）中浸泡不同时间（1、3、7 天），研究其体外生物活性，包括凝固时间、抗压强度和细胞行为。结果表明，V5 能迅速诱导羟基磷灰石（HA）的形成。通过研究细胞活力、黏附和增殖来评估 V5 对 MC3T3-E1 成骨样细胞的生物效应。CCK-8 测定表明，经过水合碳化后，V5 上的细胞活力明显提高。与 V50 水泥相比，V5 水泥增强了细胞附着、增殖和分化的更高表达。SEM 结果表明，V5 上培养的细胞的黏附和增殖有显著改善。实验结果表明，含方解石的 CS 骨水泥具有更优异的生物活性和适中的凝固时间，这表明它们在 MC3T3-E1 细胞刺激和骨组织工程等领域具有潜在的应用前景。
