Cui Qingxin, Liu Yang, Zhou Mengge, Han Yanqi, Yin Chengcheng, Bai Gang
College of Pharmacy, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China.
Front Pharmacol. 2018 Aug 21;9:948. doi: 10.3389/fphar.2018.00948. eCollection 2018.
Understanding the distribution and interaction of the Traditional Chinese Medicines (TCMs) is an integral source of herbal drug discovery. An optimized radio-labeled method was explored that could conduct biodistribution studies in animals. We evaluated the feasibility of the method and applied glycyrrhetinic acid and platycodon (PG) polysaccharides as models. [F]-GA is a novel radiotracer which was performed positron emission tomography (PET) studies to assay the biodistribution of GA in mice. In addition, PG polysaccharide was used to intervene the biodistribution and dosimetry of GA. Scanning data were analyzed with professional software. Record the time-activity curves for all organs then use the normalization method to calculate the area under the curve as a dosimetry for each organ. Moreover, the addition of PG polysaccharides can significantly improve the dosimetry of GA in the lungs, and its effect was related to the administration time. MicroPET imaging opens up a new avenue for the application of drug interactions between the TCMs.
了解中药的分布和相互作用是草药药物发现的一个不可或缺的来源。探索了一种优化的放射性标记方法，该方法可在动物体内进行生物分布研究。我们评估了该方法的可行性，并应用甘草次酸和桔梗（PG）多糖作为模型。[F]-GA是一种新型放射性示踪剂，用于进行正电子发射断层扫描（PET）研究，以测定GA在小鼠体内的生物分布。此外，PG多糖用于干预GA的生物分布和剂量测定。使用专业软件分析扫描数据。记录所有器官的时间-活性曲线，然后使用归一化方法计算曲线下面积作为每个器官的剂量测定值。此外，添加PG多糖可显著改善GA在肺部的剂量测定，其效果与给药时间有关。MicroPET成像为中药之间药物相互作用的应用开辟了一条新途径。
