Arzate M E, Morán J, Pasantes-Morales H
Neuropharmacology. 1986 Jul;25(7):689-94. doi: 10.1016/0028-3908(86)90083-3.
4-Aminopyridine (4-AP) stimulated the release of [3H]dopamine from striatal synaptosomes in the rat. At a concentration of 200 microM, 4-aminopyridine increased the spontaneous efflux of dopamine by 170%. The effect of 4-aminopyridine was calcium-dependent, being abolished when calcium was omitted from the incubation medium. Taurine, at a concentration of 25 mM, decreased the stimulatory effect of 4-aminopyridine from 170 to 49%, in the presence of 2.5 mM calcium. When the concentration of calcium in the superfusion medium was reduced to 0.1 mM, taurine had a complete inhibitory effect on the release of [3H]dopamine stimulated by 4-aminopyridine. The effect of taurine was dose-dependent. Glycine had no effect on the release of [3H]dopamine stimulated by 4-aminopyridine, either in the presence of absence of calcium, whereas gamma-aminobutyric acid (GABA) showed a slight inhibitory effect in both conditions. The results suggest that taurine antagonizes the release of [3H]dopamine induced by 4-aminopyridine through an effect mediated by calcium.
4-氨基吡啶（4-AP）刺激了大鼠纹状体突触体中[3H]多巴胺的释放。在200微摩尔的浓度下，4-氨基吡啶使多巴胺的自发流出增加了170%。4-氨基吡啶的作用依赖于钙，当孵育介质中不含钙时该作用消失。在存在2.5毫摩尔钙的情况下，25毫摩尔浓度的牛磺酸将4-氨基吡啶的刺激作用从170%降至49%。当灌注介质中的钙浓度降至0.1毫摩尔时，牛磺酸对4-氨基吡啶刺激的[3H]多巴胺释放具有完全抑制作用。牛磺酸的作用呈剂量依赖性。甘氨酸在有或无钙的情况下对4-氨基吡啶刺激的[3H]多巴胺释放均无影响，而γ-氨基丁酸（GABA）在两种情况下均显示出轻微的抑制作用。结果表明，牛磺酸通过钙介导的效应拮抗4-氨基吡啶诱导的[3H]多巴胺释放。
