Han Xiao, An Liping, Yan Dongmei, Hiroshi Matsuura, Ding Weiguang, Zhang Mengchuan, Xu Guangyu, Sun Ying, Yuan Guangxin, Wang Manli, Zhao Nanxi, Sun Jingbo, Zhu Xun, Du Peige
Department of Microbial and Biochemical Pharmacy, College of Pharmaceutical Science, Beihua University, Changchun, Jilin 132013, P.R. China.
Department of Immunology, Norman Bethune College of Medicine, Jilin University, Changchun, Jilin 130021, P.R. China.
Exp Ther Med. 2018 Sep;16(3):1586-1592. doi: 10.3892/etm.2018.6422. Epub 2018 Jul 6.
The present study has demonstrated that P-5m octapeptide (P-5m) has therapeutic potential in metastatic human hepatocarcinoma, possibly through the modulation of matrix metalloproteinase-2 expression. The purpose of the present study was to evaluate the antitumor effect of P-5m combined with 5-fluorouracil (5-Fu) on the treatment of hepatoma 22 (H22) hepatocarcinoma malignant ascites in a mouse model. The inhibitory effect on the growth of mouse ascites tumors was monitored by measuring body weight gain, survival time, ascites volume, numbers of tumor cells, DNA synthesis and peritoneal capillary permeability analysis. The present data revealed a significant reduction in ascites volume and cell count in mice that were treated with P-5m plus 5-Fu. Furthermore, the median survival time in mice in the combination group was prolonged compared with the disease control group. Moreover, a significant reduction in the total H22 ascites cell count in mice from the combination group was observed when compared with the disease control group. P-5m plus 5-Fu was able to induce the cell cycle arrest and inhibit the peritoneal capillary permeability of the mice. To conclude, the present study indicated that P-5m may have therapeutic potential in ascites caused by hepatocellular carcinoma.
本研究表明，P-5m八肽（P-5m）在转移性人类肝癌中具有治疗潜力，可能是通过调节基质金属蛋白酶-2的表达来实现的。本研究的目的是评估P-5m联合5-氟尿嘧啶（5-Fu）对小鼠肝癌22（H22）肝癌恶性腹水的治疗效果。通过测量体重增加、生存时间、腹水量、肿瘤细胞数量、DNA合成以及腹膜毛细血管通透性分析来监测对小鼠腹水肿瘤生长的抑制作用。目前的数据显示，接受P-5m加5-Fu治疗的小鼠腹水量和细胞计数显著减少。此外，联合治疗组小鼠的中位生存时间比疾病对照组延长。而且，与疾病对照组相比，联合治疗组小鼠的H22腹水细胞总数显著减少。P-5m加5-Fu能够诱导细胞周期停滞并抑制小鼠的腹膜毛细血管通透性。总之，本研究表明P-5m在肝细胞癌引起的腹水中可能具有治疗潜力。
