Huang Kui, Wu Guofeng, Zou Ji, Peng Songming
Department of Orthopaedics, The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China.
Department of Acupuncture-Moxibustion and Orthopaedics-Traumatology, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China.
Exp Ther Med. 2018 Sep;16(3):1655-1662. doi: 10.3892/etm.2018.6353. Epub 2018 Jun 26.
The advantages of combining local delivery of bone morphogenetic protein (BMP)-2 with systemic or local anti-osteoporosis treatments have also been studied for enhancing osteoporotic fracture healing. The aim of the present study was to evaluate the effect of combination therapy with BMP-2 and psoralen on fracture repair in ovariectomized mice. At 6 weeks after bilateral ovariectomy, mice (n=30) underwent unilateral transverse osteotomy on the femur and were divided into 3 groups. In the model group (n=10), animals were implanted with an absorbable collagen sponge (ACS) alone and administered physiological saline intragastrically (i.g.). In the recombinant human (rh)BMP-2 group (n=10), animals were implanted with an ACS loaded with 2.5 µg rhBMP-2 and administered physiological saline i.g. In the psoralen + rhBMP-2 group (n=10) animals were implanted with an ACS loaded with 2.5 µg rhBMP-2 and administered psoralen i.g. The mice were euthanized after 21 days and their fractured femurs were assessed by micro computed tomography, histological analysis and biomechanical testing. Furthermore, the serum of the animals was analyzed. Psoralen + rhBMP-2 exerted more beneficial effects on callus consolidation and biomechanical strength. In addition, increased bone-specific alkaline phosphatase levels and decreased C-terminal telopeptide of type-1 collagen were observed in the Psoralen + rhBMP-2 group. However, no difference in estrogen levels was detected between the groups. In conclusion, the present study demonstrated that in ovariectomized mice, combination of locally delivered BMP-2 and systemically administered psoralen improved bone healing compared with BMP-2 alone.
为增强骨质疏松性骨折的愈合，人们还研究了将骨形态发生蛋白（BMP）-2局部给药与全身或局部抗骨质疏松治疗相结合的优势。本研究的目的是评估BMP-2与补骨脂素联合治疗对去卵巢小鼠骨折修复的影响。双侧卵巢切除术后6周，30只小鼠行单侧股骨横向截骨术，并分为3组。模型组（n = 10），动物仅植入可吸收胶原海绵（ACS），并经胃内（i.g.）给予生理盐水。重组人（rh）BMP-2组（n = 10），动物植入负载2.5μg rhBMP-2的ACS，并经胃内给予生理盐水。补骨脂素+ rhBMP-2组（n = 10），动物植入负载2.5μg rhBMP-2的ACS，并经胃内给予补骨脂素。21天后对小鼠实施安乐死，并通过微型计算机断层扫描、组织学分析和生物力学测试对其骨折的股骨进行评估。此外，还对动物的血清进行了分析。补骨脂素+ rhBMP-2对骨痂巩固和生物力学强度发挥了更有益的作用。此外，在补骨脂素+ rhBMP-2组中观察到骨特异性碱性磷酸酶水平升高，1型胶原C末端肽水平降低。然而，各组之间雌激素水平未检测到差异。总之，本研究表明，在去卵巢小鼠中，与单独使用BMP-2相比，局部递送BMP-2与全身给予补骨脂素相结合可改善骨愈合。
