Huang Kui, Sun Ya-Qiong, Chen Xiao-Feng, Tian Feng, Cheng Fan, Gong Qian-Long, Liu Ke-Bin
Department of Orthopedics, The First Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China.
Department of Imaging, The First Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China.
Exp Ther Med. 2021 Apr;21(4):368. doi: 10.3892/etm.2021.9799. Epub 2021 Feb 19.
Psoralen is an effective active component extracted from , which can promote bone formation in osteoporotic animals. However, to the best of our knowledge, its effect on fracture healing has not yet been examined. In the present study, open femur fractures were created in ovariectomy (OVX)-induced osteoporotic mice. OVX mice were treated with psoralen (psoralen+OVX group) or physiological saline (OVX group) by oral gavage. Radiographic and histological results demonstrated progressed callus consolidation in the psoralen+OVX group compared with the OVX group after 10 and 21 days of treatment. Qualitative histological analysis showed that the number of osteoclasts was significantly reduced in the psoralen+OVX group after treatment. Moreover, reverse transcription-quantitative PCR analysis of callus samples showed increased expression of bone morphogenetic protein-2 (BMP-2) and osteoprotegerin (OPG), and decreased expression of receptor activator of nuclear factor-κB ligand (RANKL) at 10 and 21 days post injury in the psoralen+OVX group compared with the OVX group. Furthermore, western blot analysis showed that psoralen significantly increased the expression of estrogen receptor (ER)-α, but had no effect on ER-β expression; these results were further confirmed by immunohistochemistry. To conclude, these results indicated that psoralen may promote callus formation and inhibit osteoclast genesis by increasing BMP-2 and ER-α levels, and OPG/RANKL ratio. Consequently, psoralen could be a possible treatment for osteoporotic fracture-related complications.
补骨脂素是从[具体来源未给出]中提取的一种有效活性成分，它可以促进骨质疏松动物的骨形成。然而，据我们所知，其对骨折愈合的影响尚未得到研究。在本研究中，在卵巢切除（OVX）诱导的骨质疏松小鼠中制造开放性股骨骨折。通过灌胃给予OVX小鼠补骨脂素（补骨脂素+OVX组）或生理盐水（OVX组）。影像学和组织学结果表明，治疗10天和21天后，补骨脂素+OVX组的骨痂巩固情况比OVX组有所进展。定性组织学分析显示，治疗后补骨脂素+OVX组破骨细胞数量显著减少。此外，对骨痂样本进行的逆转录定量PCR分析表明，与OVX组相比，补骨脂素+OVX组在损伤后10天和21天时骨形态发生蛋白-2（BMP-2）和骨保护素（OPG）的表达增加，核因子κB受体活化剂配体（RANKL）的表达降低。此外，蛋白质印迹分析表明，补骨脂素显著增加雌激素受体（ER）-α的表达，但对ER-β的表达无影响；免疫组织化学进一步证实了这些结果。总之，这些结果表明，补骨脂素可能通过增加BMP-2和ER-α水平以及OPG/RANKL比值来促进骨痂形成并抑制破骨细胞生成。因此，补骨脂素可能是治疗骨质疏松性骨折相关并发症的一种可能方法。
