金环蛇蛇毒乙酰胆碱酯酶的动力学及提取物对其的抑制作用与阿尔茨海默病治疗的关系
kinetics and inhibition of krait snake's venom acetylcholinesterase by extract in relation to the treatment of Alzheimer's disease.
作者信息
Ahmed Mushtaq, Razaq Aden, Razaq Abdul, Mushtaq Nadia, Khan Rahmat Ali
机构信息
Department of Biotechnology, Faculty of Biological Sciences, University of Science & Technology, Bannu 28100, KPK-Pakistan.
Bannu Medical College Bannu, KPK, Pakistan.
出版信息
Iran J Basic Med Sci. 2018 Aug;21(8):869-872. doi: 10.22038/IJBMS.2018.28884.6979.
OBJECTIVES
The objective of this study was to evaluate pharmacological effect of against Krait snake's venom acetylcholinesterase (AChE) and to extent it for the treatment of Alzheimer's disease.
MATERIALS AND METHODS
Acetylcholinesterase activity was measured using Ellman method with some modification. The kinetic studies of methanolic extract of against krait () snake venom AChE was measured with the help of the Lineweaver Burk double reciprocal plot.
RESULTS
Statistical data of the results showed that extract inhibited the krait venom AChE in concentration dependent manner. Kinetic analysis using Line weaver Burk plot revealed that caused mixed type of inhibition i.e. km value increased (25-106.6%) while Vmax decreased from 15 to 50% with an increase of extract concentrations (100-300 µg/ml). The calculated IC value of was found to be 250 µg/ml.
CONCLUSION
extract can be considered as a therapeutic agent to cure Alzheimer's disease via inhibition of AChE activity to increase the level of acetylcholine in the body system.
目的
本研究的目的是评估[提取物名称]对金环蛇蛇毒乙酰胆碱酯酶（AChE）的药理作用，并探讨其在治疗阿尔茨海默病方面的应用。
材料与方法
采用Ellman法并稍作修改来测定乙酰胆碱酯酶活性。借助Lineweaver Burk双倒数图测定[提取物名称]甲醇提取物对金环蛇蛇毒AChE的动力学研究。
结果
结果的统计数据表明，[提取物名称]提取物以浓度依赖性方式抑制金环蛇毒AChE。使用Lineweaver Burk图进行的动力学分析表明，[提取物名称]产生混合型抑制，即随着提取物浓度（100 - 300μg/ml）的增加，km值增加（25 - 106.6%），而Vmax从15%降至50%。计算得出[提取物名称]的IC值为250μg/ml。
结论
[提取物名称]提取物可被视为一种治疗剂，通过抑制AChE活性以提高体内乙酰胆碱水平来治疗阿尔茨海默病。
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