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由类小球藻绿藻的NC - 1A病毒感染诱导的限制性内切酶活性

Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga.

作者信息

Xia Y, Burbank D E, Van Etten J L

出版信息

Nucleic Acids Res. 1986 Aug 11;14(15):6017-30. doi: 10.1093/nar/14.15.6017.

DOI:10.1093/nar/14.15.6017
PMID:3018667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311618/
Abstract

A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.

摘要

一种II型限制性内切酶CviBI是从感染了含双链DNA病毒NC-1A的类似小球藻的真核绿藻中分离出来的。该酶识别序列GANTC，并在G和A之间切割DNA。GANTC序列中的脱氧腺苷甲基化可能会抑制酶的活性。在体外，CviBI可切割宿主核DNA，但不切割病毒DNA。对另外18种感染同一小球藻属物种的病毒进行的调查显示，其中5种病毒的感染也诱导产生了一种限制性内切酶，该酶将DNA切割成与CviBI相同大小的片段。

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Nucleic Acids Res. 1985 May 24;13(10):3471-8. doi: 10.1093/nar/13.10.3471.
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Virology. 1986 Apr 15;150(1):170-7. doi: 10.1016/0042-6822(86)90276-x.
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