Zhang Y, Nelson M, Nietfeldt J W, Burbank D E, Van Etten J L
Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
Nucleic Acids Res. 1992 Oct 25;20(20):5351-6. doi: 10.1093/nar/20.20.5351.
A second DNA site-specific (restriction) endonuclease (R.CviAII) and its cognate adenine DNA methyltransferase (M.CviAII) were isolated from virus PBCV-1 infected Chlorella strain NC64A cells. R.CviAII, a heteroschizomer of the bacterial restriction endonuclease NlaIII, recognizes the sequence CATG, and does not cleave CmATG sequences. However, unlike NlaIII, which cleaves after the G and does not cleave either CmATG or mCATG sequences, CviAII cleaves between the C and A and is unaffected by mCATG methylation. The M.CviAII and R.CviAII genes were cloned and their DNA sequences were determined. These genes are tandemly arranged head-to-tail such that the TAA termination codon of the M.CviAII methyltransferase gene overlaps the ATG translational start site of R.CviAII endonuclease. R.CviAII is the first chlorella virus site-specific endonuclease gene to be cloned and sequenced.
从感染病毒PBCV-1的小球藻菌株NC64A细胞中分离出第二种DNA位点特异性（限制性）内切核酸酶（R.CviAII）及其同源腺嘌呤DNA甲基转移酶（M.CviAII）。R.CviAII是细菌限制性内切核酸酶NlaIII的异源同裂酶，识别序列CATG，不切割CmATG序列。然而，与在G后切割且不切割CmATG或mCATG序列的NlaIII不同，CviAII在C和A之间切割，不受mCATG甲基化的影响。克隆了M.CviAII和R.CviAII基因并测定了它们的DNA序列。这些基因头尾串联排列，使得M.CviAII甲基转移酶基因的TAA终止密码子与R.CviAII内切核酸酶的ATG翻译起始位点重叠。R.CviAII是第一个被克隆和测序的小球藻病毒位点特异性内切核酸酶基因。
