El Hadad Sahar, Alhebshi Alawiah, Al Amri Haifa
Pak J Biol Sci. 2018;21(1):38-50. doi: 10.3923/pjbs.2018.38.50.
Dengue is the most important arthropod-borne viral disease which caused by the four-dengue virus serotypes (1-4) and its incidence has grown dramatically around the world in the recent decades. This study was conducted to determine the molecular characterization of dengue virus genotypes spreading in Jeddah province.
To distinguish dengue virus genotypes, serum samples from 13 infected patients were subjected to envelop and non-structural 1 (E/NS1) gene amplification and sequence analysis at the nucleotide and amino acid levels.
The present partial dengue virus phylogenetic analysis announced the domination of dengue virus 2 genotype among the current dengue virus samples circulating in Jeddah province. Dengue virus 2 current isolates were grouped in one branch and seemed to be more closely related to various strains isolated from Sri-Lanka, Australia and Singapore and confirmed by internucleotide distance average ranged +/-0.01. Interestingly, sequences analysis of amino acids confirmed substitution of 8 amino acid residue (Ser729Gua, Ser729Arg, Val762Gau, Val780phe, Val781Leu, Val781Ala, Glu858Asp and Gln873His) among the present isolates comparing with previous references strains isolated from different countries. Remarkably, one unique amino acid residue Ala741Val was verified in the 10 present isolates compared to the reference sequence previously isolated from Jeddah.
Notably, the present study demonstrated the sequencing analysis of the dengue virus 2-E/NS1 on both nucleotide and amino acid levels and confirmed its endogenously prevalence in Jeddah.
登革热是由四种登革病毒血清型（1 - 4）引起的最重要的节肢动物传播病毒性疾病，近几十年来其发病率在全球范围内急剧上升。本研究旨在确定在吉达省传播的登革病毒基因型的分子特征。
为区分登革病毒基因型，对13例感染患者的血清样本进行包膜蛋白和非结构蛋白1（E/NS1）基因扩增，并在核苷酸和氨基酸水平进行序列分析。
目前的部分登革病毒系统发育分析表明，在吉达省目前流行的登革病毒样本中，登革病毒2型基因型占主导地位。登革病毒2型的当前分离株聚集在一个分支中，似乎与从斯里兰卡、澳大利亚和新加坡分离的各种毒株关系更密切，平均核苷酸距离在+/-0.01范围内得到证实。有趣的是，氨基酸序列分析证实，与先前从不同国家分离的参考毒株相比，当前分离株中有8个氨基酸残基发生了替代（Ser729Gua、Ser729Arg、Val762Gau、Val780phe、Val781Leu、Val781Ala、Glu858Asp和Gln873His）。值得注意的是，与先前从吉达分离的参考序列相比，在目前的10个分离株中验证了一个独特的氨基酸残基Ala741Val。
值得注意的是，本研究在核苷酸和氨基酸水平上对登革病毒2型E/NS1进行了测序分析，并证实了其在吉达的内源流行情况。
