Zaki Ali, Perera David, Jahan Shahrina Shan, Cardosa Mary Jane
Dr Soliman Fakeeh Hospital, Jeddah, Saudi Arabia.
Trop Med Int Health. 2008 Apr;13(4):584-92. doi: 10.1111/j.1365-3156.2008.02037.x. Epub 2008 Feb 1.
The nucleotide sequence of the 240 bp E/NS1 junction of 81 dengue viruses isolated from cases in Jeddah, Saudi Arabia was determined and used to serotype the viruses. The nucleotide sequences of the complete Envelope (E) genes of 19 isolates were used for a phylogenetic analysis of the dengue viruses circulating in Saudi Arabia from 1994 to 2006. Three of the four dengue serotypes (DENV-1, DENV-2 and DENV-3) were found to circulate, often with more than one serotype in each outbreak. There was a major outbreak caused by DENV-1 and DENV-2 in 1994 while DENV-3 emerged in 1997. In the summer of 2004, all three serotypes were isolated and this gave way to an extended outbreak of DENV-1 that stretched from the summer of 2005 through early 2006. In the 1994 outbreak, the DENV-1 circulating was from the America-Africa genotype (lineage India-2) while the most recent outbreak in 2005 and 2006 was caused by a different DENV-1 strain from genotype Asia (lineage Asia-2), suggesting a re-introduction of DENV-1 a decade after the first introduction in 1994. There has been no change in the genotypes of DENV-2 (cosmopolitan genotype) and DENV-3 (genotype III) circulating since introduction in 1994 and 1997, respectively.
测定了从沙特阿拉伯吉达病例中分离出的81株登革病毒240 bp E/NS1连接区的核苷酸序列，并用于对病毒进行血清分型。利用19株病毒完整包膜（E）基因的核苷酸序列，对1994年至2006年在沙特阿拉伯流行的登革病毒进行了系统发育分析。发现四种登革血清型中的三种（DENV-1、DENV-2和DENV-3）在流行，每次疫情中往往有不止一种血清型。1994年由DENV-1和DENV-2引起了一次大疫情，而DENV-3于1997年出现。2004年夏季，分离出了所有三种血清型，随后DENV-1爆发扩大，从2005年夏季持续到2006年初。在1994年的疫情中，流行的DENV-1来自美洲-非洲基因型（印度-2谱系），而2005年和2006年最近的疫情是由来自亚洲基因型（亚洲-2谱系）的不同DENV-1毒株引起的，这表明在1994年首次引入十年后DENV-1再次传入。自1994年和1997年分别引入以来，流行的DENV-2（世界基因型）和DENV-3（基因型III）的基因型没有变化。
