1 Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education,Key Laboratory of Reproduction and Genetic of Ningxia Hui Autonomous Region, and Department of Anatomy, Histology and Embryology, School of Basic Medical Science, Ningxia Medical University, Yinchuan, China.
Wenzhi Ma and Jia Wang contributed equally to this work.
Cell Transplant. 2018 Oct;27(10):1451-1458. doi: 10.1177/0963689718794126. Epub 2018 Sep 6.
Safety is the chief consideration in recipient preparation of spermatogonial stem cell (SSC) transplantation in mammals, especially humans. In this study, we compared the safety of the SSC transplantation recipients that were prepared both by testes heat shock plus testes busulfan injection (heat shock+busulfan(t)) and by busulfan intraperitoneal injection (busulfan i.p.) only. Our results showed that heat shock+busulfan(t) treatment significantly ( p < 0.05) reduced mortality in mice and did not produce bone marrow cell toxicity. Furthermore, heat shock+busulfan(t) treatment directly damaged SSCs and exhausted almost all of the germ cells in the testis; the exhaustion of these cells is considered a key factor in the successful preparation of the recipients. Therefore, we used heat shock+busulfan(t) treatment to prepare recipients of SSC transplantation. Two months after SSC transplantation, the number and length of donor SSC-derived colonies in the testis of recipient in heat shock+busulfan(t) group was closed to that in busulfan i.p. group. Therefore, compared with busulfan i.p. treatment, heat shock+busulfan(t) treatment improved the safety of recipient preparation without reducing the efficiency of SSC transplantation. Two GFP-positive offspring were produced from 1 of the 20 recipients that had mated with female mice 72 days after SSC transplantation. In conclusion, heat shock with busulfan treatment is a safe method to prepare the recipient of SSC transplantation in mice.
安全是哺乳动物，尤其是人类，准备精原干细胞（SSC）移植时的首要考虑因素。在这项研究中，我们比较了通过睾丸热休克加睾丸白消安注射（热休克+白消安（t））和仅通过白消安腹腔注射（白消安 i.p.）两种方法制备 SSC 移植受体的安全性。我们的结果表明，热休克+白消安（t）处理显著（p<0.05）降低了小鼠的死亡率，且不会产生骨髓细胞毒性。此外，热休克+白消安（t）处理直接损伤 SSCs 并耗尽睾丸中几乎所有的生殖细胞；这些细胞的耗竭被认为是成功制备受体的关键因素。因此，我们使用热休克+白消安（t）处理来制备 SSC 移植受体。在 SSC 移植后 2 个月，热休克+白消安（t）组受体睾丸中供体 SSC 衍生集落的数量和长度接近白消安 i.p.组。因此，与白消安 i.p.处理相比，热休克+白消安（t）处理在不降低 SSC 移植效率的情况下提高了受体制备的安全性。在 SSC 移植后 72 天与雌性小鼠交配的 20 只受体中，有 1 只产生了 2 只 GFP 阳性后代。总之，用白消安处理热休克是一种安全的方法，可以在小鼠中制备 SSC 移植受体。
