Acuña Francisco, Tano de la Hoz María F, Díaz Alcira O, Portiansky Enrique L, Barbeito Claudio G, Flamini Mirta A
Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata (LHYEDEYC, FCV-UNLP), La Plata, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Reprod Domest Anim. 2019 Feb;54(2):207-215. doi: 10.1111/rda.13333. Epub 2018 Sep 26.
This study reports the histochemistry and the distribution of glycoconjugates (GCs) in the zona pellucida (ZP) of preantral, secondary, tertiary, polyovulatory and atretic follicles of ovaries from non-pregnant (NPr) and pregnant (Pr) females of Lagostomus maximus. GCs were studied using histochemical and lectin histochemical methods. The viscacha ZP was positive to all the histochemical techniques. In addition, it was observed that the intensity of staining of the ZP was constant in the different follicular stages between both female groups. The lectin histochemical study revealed that ZP was positive for certain lectins (WGA, RCA-I and CON-A) and that the labelling did not vary between the different follicular stages, but between the two groups of females. By using both histochemical techniques, it was established that the GCs present in the ZP label the complexity of the area. These results allow us to increase our knowledge on the biology of the viscacha's ovary, particularly contributing to the study of polyovulation.
本研究报告了在未怀孕（NPr）和怀孕（Pr）的大毛丝鼠雌性卵巢的窦前卵泡、次级卵泡、三级卵泡、多排卵卵泡和闭锁卵泡的透明带（ZP）中糖缀合物（GCs）的组织化学和分布情况。使用组织化学和凝集素组织化学方法研究了GCs。毛丝鼠的ZP对所有组织化学技术均呈阳性。此外，观察到两组雌性不同卵泡阶段的ZP染色强度是恒定的。凝集素组织化学研究表明，ZP对某些凝集素（WGA、RCA-I和CON-A）呈阳性，并且标记在不同卵泡阶段之间没有变化，但在两组雌性之间存在差异。通过使用这两种组织化学技术，确定了ZP中存在的GCs标记了该区域的复杂性。这些结果使我们能够增加对毛丝鼠卵巢生物学的了解，特别是有助于多排卵的研究。
