Terra Juliano P, Almeida Thiago B, Rezende Lucas P O, Barbeito Claudio G, Acuña Francisco, Blume Guilherme R, Eloi Rômulo S A, Oliveira Letícia B, Santos André L R M, Sant'Ana Fabiano J F
Laboratório de Diagnóstico Patológico Veterinário (LDPV), Universidade de Brasília (UnB), Brasília, Brazil.
Facultad de Ciencias Veterinarias (FCV), Universidad Nacional de La Plata (UNLP), La Plata, Argentina.
Reprod Domest Anim. 2025 May;60(5):e70075. doi: 10.1111/rda.70075.
Studies have demonstrated that many reproductive disorders can compromise the binding pattern of glycosidic residues, mainly in cows and sows. In the current study, the binding pattern of lectins was characterised and compared in normal and pathological ovaries of bitches. Fourteen biotinylated lectins (BSA-1B, Con-A, DBA, LCA, PHA-E, PHA-L, PNA, RCA-1, SBA, SJA, STA, WGA, s-WGA, UEA-1) were used. In ovaries without lesions, the predominant staining for RCA-1 was observed in the zona pellucida (ZP) of initial follicles, in the oocytes of primordial and primary follicles, and in the artery wall. For WGA, the reaction predominated in the mesothelium, in the ZP of secondary follicles, in the corpus luteum (CL) and in the oocyte of secondary follicles. Reactivity for STA was more pronounced in the mesothelium and ZP of developing follicles. The mesothelial cells and the CL were mainly marked for LCA. PHA-L was stained in the mesothelium, CL, and connective stroma, while the lectin PHA-E showed reactivity especially in the stroma, mesothelium, and artery wall. In the cystic ovaries, changes in the reaction of some lectins were noted in various structures adjacent to the cysts. RCA-1 staining was reduced in the ZP and oocyte of primordial, primary, and secondary follicles, while there was an increase of staining in endothelial cells. With WGA lectin, there was a reduction in CL and an increase in endothelial cells. Reactivity to STA was reduced in the ZP of primary and secondary follicles and increased in endothelial cells. LCA was also reduced in the CL and ZP of primary and secondary follicles. For the PHA-L lectin, the staining was reduced in the mesothelial cells, CL, and ZP of the primary follicles, while for PHA-E the reduction occurred in the stroma, oocyte of the primary follicles, and in the GC of the secondary follicles. In all neoplasms analysed (dysgerminoma, granulosa cell tumour, adenocarcinoma), there was also a reduction in reactivity to various lectins, both in the neoplastic cells compared to the cells of origin and in the adjacent non-neoplastic ovarian tissue. The data from the current study suggest that the changes in ovarian glycosidic residues observed in cysts and neoplasms may contribute to infertility in bitches.
研究表明，许多生殖系统疾病会破坏糖苷残基的结合模式，主要发生在奶牛和母猪身上。在本研究中，对正常和病理状态下母犬卵巢中凝集素的结合模式进行了表征和比较。使用了十四种生物素化凝集素（BSA-1B、Con-A、DBA、LCA、PHA-E、PHA-L、PNA、RCA-1、SBA、SJA、STA、WGA、s-WGA、UEA-1）。在无病变的卵巢中，观察到RCA-1在初始卵泡的透明带（ZP）、原始卵泡和初级卵泡的卵母细胞以及动脉壁中主要染色。对于WGA，反应主要在间皮、次级卵泡的ZP、黄体（CL）和次级卵泡的卵母细胞中。STA在发育卵泡的间皮和ZP中的反应更明显。间皮细胞和CL主要被LCA标记。PHA-L在间皮、CL和结缔组织基质中染色，而凝集素PHA-E尤其在基质、间皮和动脉壁中显示出反应性。在囊性卵巢中，在囊肿相邻的各种结构中观察到一些凝集素反应的变化。RCA-1在原始卵泡、初级卵泡和次级卵泡的ZP和卵母细胞中的染色减少，而内皮细胞中的染色增加。对于WGA凝集素，CL减少，内皮细胞增加。对STA的反应性在初级和次级卵泡的ZP中降低，在内皮细胞中增加。LCA在初级和次级卵泡的CL和ZP中也减少。对于PHA-L凝集素，初级卵泡的间皮细胞、CL和ZP中的染色减少，而对于PHA-E，减少发生在基质、初级卵泡的卵母细胞和次级卵泡的颗粒细胞（GC）中。在所有分析的肿瘤（无性细胞瘤、颗粒细胞瘤、腺癌）中，与起源细胞相比，肿瘤细胞以及相邻的非肿瘤性卵巢组织中对各种凝集素的反应性也降低。本研究的数据表明，在囊肿和肿瘤中观察到的卵巢糖苷残基变化可能导致母犬不孕。
