Dobesová Z, Mourek J
Physiol Bohemoslov. 1986;35(3):277-80.
The outer membrane resistance of brain and liver mitochondria to the action of digitonin was studied in 5- and 60-day-old rats. Disintegration of the outer membrane was evaluated by means of cytochrome oxidase activity. The mitochondria were isolated by the Ficoll gradient method. Brain mitochondria were found to be significantly more resistant to digitonin than liver mitochondria. It was also demonstrated that the resistance of the outer membrane of both brain and liver mitochondria rose approximately 1.3- to 1.4-fold during ontogenesis.
在5日龄和60日龄大鼠中研究了脑和肝线粒体的外膜对洋地黄皂苷作用的抗性。通过细胞色素氧化酶活性评估外膜的解体情况。采用Ficoll梯度法分离线粒体。发现脑线粒体对洋地黄皂苷的抗性明显高于肝线粒体。还证明了在个体发育过程中，脑和肝线粒体的外膜抗性均提高了约1.3至1.4倍。
