Nosrati Hamed, Hamzehei Hossein, Afroogh Saeed, Ashabi Seyedeh Fatemeh, Attari Elahe, Manjili Hamidreza Kheiri
Student Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Pharmaceutical Biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Drug Res (Stuttg). 2019 May;69(5):277-283. doi: 10.1055/a-0664-0431. Epub 2018 Sep 6.
In this study we reported the synthesis of L-phenyl alanine (Phe) & L-tyrosine (Tyr) Natural Amino acids coated iron oxide magnetic nanoparticles under one-pot and in situ reaction. Functionalized iron oxide magnetic nanoparticles were characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Vibrating Sample Magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) techniques. Cellular toxicity of amino acids coated iron oxide magnetic nanoparticles was also investigated on HEK-293 cell lines. Additionally, a hemolysis test of as prepared magnetic nanoparticles were performed. It was found that the synthesized Phe and Tyr coated magnetic nanoparticles (F@Phe NPs and F@Tyr NPs) were spherical in shape with an average size less than 25 nm, also the saturation magnetization (Ms) of the F@Phe NPs and F@Tyr NPs were about 30.02 and 58.23 emu/g, respectively, which was lower than those of bare FeO. The TGA results show that apart from this weight loss, the coated sample shows a weight loss of 5.48, and 6.88% respectively corresponding to loss of Tyr, and Phe which is coated on the FeO nanoparticles. At a high concentration, less than 2.92 and 3.13% hemolytic activity were observed for F@Phe NPs and F@Tyr NPs, respectively. The F@Phe NPs and F@Tyr NPs show the possibility of using this nanoparticles in the development of in vitro and in vivo pharmaceutical and biomedical fields due to do not possess a toxic effect, good ζ-potential and related small and narrow size distribution.
在本研究中，我们报道了通过一锅原位反应合成L-苯丙氨酸（Phe）和L-酪氨酸（Tyr）包覆的天然氨基酸氧化铁磁性纳米颗粒。通过X射线衍射（XRD）、差示扫描量热法（DSC）、热重分析（TGA）、振动样品磁强计（VSM）、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）技术对功能化的氧化铁磁性纳米颗粒进行了表征。还研究了氨基酸包覆的氧化铁磁性纳米颗粒对HEK-293细胞系的细胞毒性。此外，对制备的磁性纳米颗粒进行了溶血试验。结果发现，合成的Phe和Tyr包覆磁性纳米颗粒（F@Phe NPs和F@Tyr NPs）呈球形，平均尺寸小于25nm，F@Phe NPs和F@Tyr NPs的饱和磁化强度（Ms）分别约为30.02和58.23 emu/g，低于裸FeO的饱和磁化强度。TGA结果表明，除了这种重量损失外，包覆样品分别显示出5.48%和6.88%的重量损失，这对应于包覆在FeO纳米颗粒上的Tyr和Phe的损失。在高浓度下，F@Phe NPs和F@Tyr NPs的溶血活性分别低于2.92%和3.13%。由于F@Phe NPs和F@Tyr NPs不具有毒性作用、良好的ζ电位以及相关的小且窄的尺寸分布，它们显示出在体外和体内制药及生物医学领域开发中使用这种纳米颗粒的可能性。
