通过超顺磁 Fe₃O₄ 磁性胶体纳米晶簇提取基因组 DNA。
Extraction of Genomic DNA via Superparamagnetic Fe₃O₄ Magnetic Colloidal Nanocrystal Clusters.
机构信息
School of Life Science, Shihezi University, Xinjiang 832003, P. R. China.
出版信息
J Nanosci Nanotechnol. 2018 Dec 1;18(12):8105-8110. doi: 10.1166/jnn.2018.16399.
We herein describe a solvothermal method for preparing superparamagnetic Fe3O4 magnetic colloidal nanocrystal clusters with an average diameter of 195 nm. The Fe3O4 magnetic colloidal nanocrystal clusters were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and other methods. The Fe3O4 magnetic colloidal nanocrystal clusters were used to extract genomic DNA from fresh blood, Gram-negative bacteria, Gram-positive bacteria and frozen blood. Results showed that the genomic DNA integrity, purity and yield extracted via this universal method were satisfactory. This method is rapid, simple and does not use protease K or toxic compounds. This method lays the foundation for future research and nucleic acid extraction techniques.
我们在此描述了一种溶剂热法，用于制备平均直径为 195nm 的超顺磁性 Fe3O4 磁性胶体纳米晶簇。通过透射电子显微镜（TEM）、动态光散射（DLS）等方法对 Fe3O4 磁性胶体纳米晶簇进行了表征。Fe3O4 磁性胶体纳米晶簇用于从新鲜血液、革兰氏阴性菌、革兰氏阳性菌和冷冻血液中提取基因组 DNA。结果表明，通过这种通用方法提取的基因组 DNA 完整性、纯度和产量令人满意。该方法快速、简单，不使用蛋白酶 K 或有毒化合物。该方法为未来的研究和核酸提取技术奠定了基础。
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