Kumar Mukul, Kaushik Deepika, Kumar Ashwani, Krishnan Hari, Oz Fatih, Proestos Charalampos, Hashem Abeer, Abd Allah Elsayed Fathi
Department of Food Technology and Nutrition, Lovely Professional University, Phagwara, 144411, Punjab, India.
Department of Biotechnology, Faculty of Applied Science and Biotechnology, Shoolini University, Solan, H.P., 173229, India.
Heliyon. 2024 Apr 12;10(8):e29433. doi: 10.1016/j.heliyon.2024.e29433. eCollection 2024 Apr 30.
Nanoparticles have different shapes and sizes between the range of 1-100 nm, which show advantages for stabilizing compounds, higher carrier capacity, and lower costs. Metal nanoparticles such as copper, gold, silver, and zinc are favorable components for various applications due to their interesting properties. In the present study, nanoparticles were synthesized by reduction with flower extracts of & that were used to stabilize the copper nanoparticles. Furthermore, the characterization of plants synthesized copper nanoparticles was carried out through UV-visible dynamic light scattering. Additionally, morphological characterization of nanoparticles was confirmed by scanning electron microscopy and energy dispersive X-ray spectroscopy confirmed the elemental composition of copper nanoparticles. Powder X-ray diffraction was conducted for the analysis of crystallinity, purity, and crystal size of plant-synthesized copper nanoparticles. The average particle size was evaluated and exhibited the particle size at the peak of 8.721 nm and 98.03 nm for flower extracts of & copper nanoparticles. The Fourier Transform Infrared spectrum was taken to scrutinize the various functional groups that were responsible for the reduction of the copper ions. The antimicrobial results against the bacterial strains with the positive test results of the zone of inhibition were for (17 mm, 18 mm, 19 mm, and 18 mm) and (17 mm, 19 mm, 18 mm, and 18 mm) respectively for plants synthesized copper nanoparticles against the , , and Lipase inhibition assay and Amylase inhibition assay with different concentrations (20 μg/mL to 100 μg/mL) for & (12.34 %-59.67 % and 10.50 %-47.01 %) and (34.52 %-89.02 % and 22.34 %-56.45 %) confirmed the anti-obesity and anti-diabetic activities of plants extract synthesized copper nanoparticles.
纳米颗粒的形状和大小各异,范围在1至100纳米之间,在稳定化合物、提高载药量和降低成本方面具有优势。诸如铜、金、银和锌等金属纳米颗粒因其有趣的特性而成为各种应用的理想成分。在本研究中,通过用[植物名称1]和[植物名称2]的花提取物还原合成纳米颗粒,这些提取物用于稳定铜纳米颗粒。此外,通过紫外可见动态光散射对植物合成的铜纳米颗粒进行表征。另外,通过扫描电子显微镜确认纳米颗粒的形态特征,能量色散X射线光谱法确定了铜纳米颗粒的元素组成。进行粉末X射线衍射分析植物合成的铜纳米颗粒的结晶度、纯度和晶体尺寸。评估了平均粒径,[植物名称1]和[植物名称2]花提取物合成的铜纳米颗粒在峰值处的粒径分别为8.721纳米和98.03纳米。采用傅里叶变换红外光谱来仔细研究负责还原铜离子的各种官能团。对具有抑菌圈阳性测试结果的细菌菌株的抗菌结果分别为,[植物名称1]合成的铜纳米颗粒对[细菌名称1]、[细菌名称2]、[细菌名称3]和[细菌名称4]的抑菌圈直径为(17毫米、18毫米、19毫米和18毫米),[植物名称2]合成的铜纳米颗粒对上述细菌的抑菌圈直径为(17毫米、19毫米、18毫米和18毫米)。用不同浓度(20微克/毫升至100微克/毫升)对[植物名称1]和[植物名称2]进行脂肪酶抑制试验和淀粉酶抑制试验,结果分别为(12.34%-59.67%和10.50%-47.01%)以及(34.52%-89.02%和22.34%-56.45%),这证实了植物提取物合成的铜纳米颗粒具有抗肥胖和抗糖尿病活性。