Gibson J G, McCue J P
Transfusion. 1978 Sep-Oct;18(5):524-9. doi: 10.1046/j.1537-2995.1978.18579036379.x.
The effect of phosphate buffer on the course of pH, ATP, and 2,3-PDG of CPD red blood cells stored at three temperatures was observed. Basic phosphate at an equilibrated level of 10 mM (as iP) maintained pH above 7.00 and ATP and 2,3-DPG above 70 per cent of initial value in cells stored at 37 C for 24 hours. In contrast however, at 25 and 4 C no buffering was obtained with basic phosphate concentrations up to 50 mM, but values for both ATP and 2,3-DPG were higher in phosphate treated aliquots than in controls throughout storage. When the pH of blood stored at 4 C was adjusted into the range 7.15 to 7.25 with tromethamine and the level of iP raised to 10 mM by addition of Na2HPO4 on day seven, it was found that ATP and 2,3-DPG levels were maintained at 90 and 120 per cent, while control levels fell to 60 and 12 per cent, respectively at 21 days. The process described parallels the normal repair of damaged red blood cells of bank blood that occurs in vivo following transfusion.
观察了磷酸盐缓冲液对在三种温度下储存的CPD红细胞的pH值、ATP和2,3 - 二磷酸甘油酸（2,3 - PDG）变化过程的影响。在37℃储存24小时的细胞中，平衡水平为10 mM（以无机磷计）的碱性磷酸盐可使pH值维持在7.00以上，ATP和2,3 - 二磷酸甘油酸（2,3 - DPG）维持在初始值的70%以上。然而，相比之下，在25℃和4℃时，高达50 mM的碱性磷酸盐浓度未获得缓冲效果，但在整个储存过程中，经磷酸盐处理的等分试样中ATP和2,3 - DPG的值均高于对照组。当在第7天用三羟甲基氨基甲烷将4℃储存血液的pH值调节至7.15至7.25范围内，并通过添加Na2HPO4将无机磷水平提高到10 mM时，发现ATP和2,3 - DPG水平在21天时分别维持在90%和120%，而对照组水平分别降至60%和12%。所描述的过程与输血后体内发生的库存血受损红细胞的正常修复过程相似。
