Dawson R B
Transfusion. 1976 Sep-Oct;16(5):450-4. doi: 10.1046/j.1537-2995.1976.16577039301.x.
Inorganic phosphate has been known to assist red blood cell maintenance of ATP and in the presence of inosine to assist in the maintenance of 2,3-DPG. High concentrations of phosphate, while helping ATP maintenance, were found to be deleterious to 2,3-DPG maintenance in CPD-adenine preservatives. However, in the presence of inosine, concentrations of phosphate as high as 10 mM were advantageous to 2,3-DPG maintenance. The present study extends the observations on ATP and 2,3-DPG maintenance in CPD-adenine-inosine preservatives from the previous 10 mM to 20 mM phosphate. A high phosphate (20 mM) effect has been seen as improved maintenance of 2,3-DPG levels during the fifth and sixth weeks of storage of whole blood at 4C. This supports the previously reported observation of improved maintenance of 2,3-DPG in a 10 mM phosphate preservative. This is ten times the 2 mM phosphate concentration in CPD-adenine. In the low phosphate preservative (2 mM), 2,3-DPG maintenance is less than that in all of the higher phosphate preservatives after the second week of storage. ATP concentrations in this experiment show good maintenance throughout six weeks of storage.
已知无机磷酸盐有助于维持红细胞中的三磷酸腺苷（ATP），并且在肌苷存在的情况下有助于维持2,3 - 二磷酸甘油酸（2,3 - DPG）。高浓度的磷酸盐虽然有助于维持ATP，但在CPD - 腺嘌呤保存液中却对2,3 - DPG的维持有害。然而，在肌苷存在的情况下，高达10 mM的磷酸盐浓度对2,3 - DPG的维持是有利的。本研究将之前关于CPD - 腺嘌呤 - 肌苷保存液中ATP和2,3 - DPG维持情况的观察范围从10 mM磷酸盐扩展到了20 mM磷酸盐。已经观察到高磷酸盐（20 mM）效应可改善全血在4℃储存的第五周和第六周期间2,3 - DPG水平的维持情况。这支持了之前报道的在10 mM磷酸盐保存液中2,3 - DPG维持情况得到改善的观察结果。这是CPD - 腺嘌呤中2 mM磷酸盐浓度的10倍。在低磷酸盐保存液（2 mM）中，储存第二周后2,3 - DPG的维持情况不如所有更高磷酸盐浓度的保存液。本实验中ATP浓度在整个六周的储存过程中都保持良好。
