Andreenkov O V, Andreenkova N G, Volkova E I, Georgiev P G, Goncharova A A, Pokholkova G V, Demakov S A
Tsitologiia. 2016;58(6):493-7.
Chromatin insulator proteins are one of the major components that determine the domain organization of the genome. According to the latest data, they can mark the boundaries of topological domains and prevent the spread of silent chromatin to adjacent areas. One approach to the analysis of the actions of these proteins is to use the ectopic involvement in the UAS>DBD(GAL4). The method allows to evaluate the effect of selected protein in chromatin organization, to establish its association with other insulator proteins and influence on the processes of transcription and replication. and influence the processes of transcription and replication. In this study, we have developed and tested the functionality of the system components in ectopic tethering of the Chromator (Chriz) to the region of intercalary heterochromatin 10A1-2. Preliminary data have been obtained showing that ectopically tethered Chromator to the band 10A1-2 can induce a partial decompactization of the band chromatin. Further use of this experimental model provides the opportunity to investigate the effect of insulator proteins on the chromatin structure.
染色质绝缘子蛋白是决定基因组结构域组织的主要成分之一。根据最新数据，它们可以标记拓扑结构域的边界，并防止沉默染色质扩散到相邻区域。分析这些蛋白质作用的一种方法是利用其异位参与UAS>DBD（GAL4）。该方法可以评估所选蛋白质在染色质组织中的作用，确定其与其他绝缘子蛋白的关联以及对转录和复制过程的影响。在本研究中，我们开发并测试了将染色质结合蛋白（Chriz）异位拴系到居间异染色质10A1-2区域的系统组件的功能。已获得初步数据，表明将Chriz异位拴系到10A1-2带可诱导该带染色质的部分解压缩。进一步使用该实验模型为研究绝缘子蛋白对染色质结构的影响提供了机会。
