ECTOPIC TETHERING OF THE CHROMATOR PROTEIN IN UASDBD(GAL4) SYSTEM AS APPROACH FOR STUDYING OF THE INSULATOR PROTEINS IN DROSOPHILA MELANOGASTER POLYTENE CHROMOSOMES.

Chromatin insulator proteins are one of the major components that determine the domain organization of the genome. According to the latest data, they can mark the boundaries of topological domains and prevent the spread of silent chromatin to adjacent areas. One approach to the analysis of the actions of these proteins is to use the ectopic involvement in the UAS>DBD(GAL4). The method allows to evaluate the effect of selected protein in chromatin organization, to establish its association with other insulator proteins and influence on the processes of transcription and replication. and influence the processes of transcription and replication. In this study, we have developed and tested the functionality of the system components in ectopic tethering of the Chromator (Chriz) to the region of intercalary heterochromatin 10A1-2. Preliminary data have been obtained showing that ectopically tethered Chromator to the band 10A1-2 can induce a partial decompactization of the band chromatin. Further use of this experimental model provides the opportunity to investigate the effect of insulator proteins on the chromatin structure.