Department of Periodontology, School of Dentistry, National Defense Medical, Center and Tri-Service General Hospital, Taipei, Taiwan.
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
J Periodontol. 2019 Mar;90(3):271-280. doi: 10.1002/JPER.16-0708. Epub 2018 Sep 27.
This study evaluated the ameliorative effect of hesperidin (HES), an anti-inflammatory flavanone, in rats with ligation (Lig)-induced periodontitis.
A total of 48 rats were randomly divided into non-ligation group (NL), Lig group, and two ligation-plus-HES groups (L+H). HES was administered immediately after ligature placement at a dose of 75 or 150 mg/kg by intragastric feeding. Destruction of the ligated maxillary second and mandibular first molars were evaluated by dental radiography, microcomputed tomography (micro-CT), and histometry performed after sacrificing the rats on the seventh day. The expression levels of interleukin (IL)-1β, IL-6, and inducible nitric oxide synthase (iNOS) messenger (m)RNAs in the gingiva were determined by reverse-transcription polymerase chain reaction. The expression of iNOS was examined by immunohistochemistry.
The dental radiography and micro-CT findings revealed significantly increased alveolar bone loss in the Lig group, which was significantly prevented by HES. The histometry results revealed less gingival inflammation and connective tissue loss in the L+H groups compared with that in the Lig group. The mRNA expression levels of IL-6, IL-1 β, and iNOS were significantly increased in the Lig group but were reduced in the L+H groups. The immunostaining results showed that the ligation-induced iNOS expression was also decreased by HES.
Oral administration of HES promotes an ameliorative effect against the ligation-induced alveolar bone loss and effectively inhibits the production of proinflammatory mediators in rats with experimentally induced periodontitis. Therefore, HES may be a good candidate for modulating oral inflammatory diseases.
本研究评估了具有抗炎作用的橙皮苷（HES）在结扎诱导的牙周炎大鼠中的改善作用。
将 48 只大鼠随机分为非结扎组（NL）、结扎组和两个结扎加 HES 组（L+H）。结扎后立即通过灌胃给予 HES，剂量为 75 或 150mg/kg。在第 7 天处死大鼠后，通过牙片、微计算机断层扫描（micro-CT）和组织计量评估结扎上颌第二磨牙和下颌第一磨牙的破坏情况。通过逆转录聚合酶链反应测定龈组织中白细胞介素（IL）-1β、IL-6 和诱导型一氧化氮合酶（iNOS）信使（m）RNA 的表达水平。通过免疫组织化学检查 iNOS 的表达。
牙片和 micro-CT 结果显示结扎组牙槽骨丢失明显增加，HES 可显著预防这种情况。组织计量学结果显示，与结扎组相比，L+H 组的牙龈炎症和结缔组织丧失较少。Lig 组的 IL-6、IL-1β和 iNOS mRNA 表达水平显著升高，但 L+H 组的表达水平降低。免疫组化结果显示，HES 还可降低结扎诱导的 iNOS 表达。
口服 HES 可促进结扎诱导的牙槽骨丢失的改善作用，并有效抑制实验性牙周炎大鼠促炎介质的产生。因此，HES 可能是调节口腔炎症性疾病的良好候选药物。
