Abraham G, Low P S
Biochim Biophys Acta. 1980 Apr 10;597(2):285-91. doi: 10.1016/0005-2736(80)90106-6.
Two new fluorescent labelling techniques are described: one specific for sialic acid residues and a second specific for galactose and some of its derivatives. Using either technique it is possible to label covalently the desired carbohydrate residue with any one of a large variety of fluorescent probes. When the sialic acid labelling procedure is applied to human erythrocyte membranes, only the glycophorin species are labelled. However, when the galactose-directed labelling scheme is applied, fluorescence is also observed on membrane lipid components and on band 3. For each technique, the fluorescent labelling pattern is shown to reflect the distribution of the respective sugar component on the erythrocyte membrane. Thus, these techniques should provide both selectivity and versatility in the fluorescent labelling of specific carbohydrate residues in highly heterogeneous biological systems.
一种对唾液酸残基具有特异性，另一种对半乳糖及其某些衍生物具有特异性。使用这两种技术中的任何一种，都可以用多种荧光探针中的任何一种将所需的碳水化合物残基共价标记。当将唾液酸标记程序应用于人红细胞膜时，只有血型糖蛋白被标记。然而，当应用半乳糖导向的标记方案时，在膜脂质成分和带3上也观察到荧光。对于每种技术，荧光标记模式都显示出反映了红细胞膜上各自糖成分的分布。因此，这些技术在高度异质的生物系统中对特定碳水化合物残基的荧光标记应具有选择性和通用性。
