Mendeleum - Institute of Genetics, Mendel University in Brno, Valtická 334, 691 44, Lednice, Czech Republic.
Department of Fruit Growing, Mendel University in Brno, Valtická 337, 691 44, Lednice, Czech Republic.
Microb Ecol. 2019 Apr;77(3):664-675. doi: 10.1007/s00248-018-1250-9. Epub 2018 Sep 7.
"Candidatus Phytoplasma prunorum" (CPp) is a highly destructive phytopathogenic agent in many stone fruit-growing regions in Europe and the surrounding countries. In this work, we focused on documenting entire bacterial community in the phloem tissues of 60 stone fruit trees. Nested PCR and two real-time PCR assays were used to select CPp-positive (group A) and CPp-negative samples (group B). Afterwards, high-throughput amplicon sequencing was performed to assess bacterial community compositions in phloem tissues. The bacterial composition in phloem tissue consisted of 118 distinct genera, represented mainly by Pseudomonas, Acinetobacter, Methylobacterium, Sphingomonas, and Rhizobium. Statistics showed that CPp influenced the bacterial composition of infected plants (group A) and that the bacterial community depended on the geographical origin of the sample. This is the first work focusing on an analysis of the influence of CPp on the bacteria coexisting in the phloem tissues of stone fruit trees.
“李树韧皮部杆菌”（CPp）是欧洲和周边国家许多核果种植区极具破坏性的植物病原物。在这项工作中，我们专注于记录 60 棵核果树上韧皮部组织中的整个细菌群落。巢式 PCR 和两种实时 PCR 检测被用于选择 CPp 阳性（A 组）和 CPp 阴性的样本（B 组）。随后，高通量扩增子测序被用于评估韧皮部组织中的细菌群落组成。韧皮部组织中的细菌组成包括 118 个不同的属，主要由假单胞菌、不动杆菌、甲基杆菌、鞘氨醇单胞菌和根瘤菌组成。统计数据表明，CPp 影响了感染植物（A 组）的细菌组成，并且细菌群落取决于样本的地理来源。这是首次聚焦于分析 CPp 对核果树枝条韧皮部中共存细菌的影响的工作。
