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基于宏基因组的转基因葡萄砧木对其相关病毒组和土壤细菌组影响的研究。

Metagenomic-based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome.

机构信息

INRA, SVQV UMR-A 1131, Université de Strasbourg, Colmar, France.

Laboratoire Ampère (CNRS UMR5005), Environmental Microbial Genomics, École Centrale de Lyon, Université de Lyon, Ecully, France.

出版信息

Plant Biotechnol J. 2018 Jan;16(1):208-220. doi: 10.1111/pbi.12761. Epub 2017 Aug 9.

DOI:10.1111/pbi.12761
PMID:28544449
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5785345/
Abstract

For some crops, the only possible approach to gain a specific trait requires genome modification. The development of virus-resistant transgenic plants based on the pathogen-derived resistance strategy has been a success story for over three decades. However, potential risks associated with the technology, such as horizontal gene transfer (HGT) of any part of the transgene to an existing gene pool, have been raised. Here, we report no evidence of any undesirable impacts of genetically modified (GM) grapevine rootstock on its biotic environment. Using state of the art metagenomics, we analysed two compartments in depth, the targeted Grapevine fanleaf virus (GFLV) populations and nontargeted root-associated microbiota. Our results reveal no statistically significant differences in the genetic diversity of bacteria that can be linked to the GM trait. In addition, no novel virus or bacteria recombinants of biosafety concern can be associated with transgenic grapevine rootstocks cultivated in commercial vineyard soil under greenhouse conditions for over 6 years.

摘要

对于某些作物来说，获得特定性状的唯一可能方法是进行基因组修饰。基于病原衍生抗性策略开发抗病毒转基因植物已经取得了三十多年的成功。然而，该技术相关的潜在风险，如转基因任何部分的水平基因转移（HGT）到现有的基因库中，已经被提出。在这里，我们没有报告任何关于转基因（GM）葡萄砧木对其生物环境产生不良影响的证据。我们使用最先进的宏基因组学技术，深入分析了两个部分，即目标葡萄扇叶病毒（GFLV）种群和非目标根相关微生物群。我们的结果显示，与 GM 特性相关的细菌遗传多样性没有统计学上的显著差异。此外，在温室条件下种植超过 6 年的商业化葡萄园土壤中，与转基因葡萄砧木相关的，具有生物安全性的新病毒或细菌重组体也不能被发现。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/11388491/6dd03a9f52d4/PBI-16-208-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/11388491/8f0eb7a7ed94/PBI-16-208-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/11388491/fa1d419d44d2/PBI-16-208-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/11388491/49d012a987be/PBI-16-208-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/11388491/7fb949cc27d0/PBI-16-208-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/11388491/6dd03a9f52d4/PBI-16-208-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/11388491/8f0eb7a7ed94/PBI-16-208-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/11388491/fa1d419d44d2/PBI-16-208-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/11388491/49d012a987be/PBI-16-208-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/11388491/7fb949cc27d0/PBI-16-208-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/11388491/6dd03a9f52d4/PBI-16-208-g001.jpg

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