College of Biological Science and Engineering, Fuzhou University, Fuzhou 350116, PR China.
MOE Key Laboratory for Analytical Science of Food Safety and Biology, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350116, PR China.
Biosens Bioelectron. 2018 Nov 30;120:137-143. doi: 10.1016/j.bios.2018.08.046. Epub 2018 Sep 5.
Cholesterol is an essential compound for maintaining cellular homeostasis and human healthy. Sensitive detection of cholesterol and efficient elimination of excess cholesterol have become the essential manipulations in clinical diagnosis and health management. To date, it is still quite challenging that cholesterol detection and elimination tasks are carried out simultaneously. In this study, bifunctional magnetic nanoparticles (FeO@PDA-PBA-CD) are designed and fabricated to overcome this difficulty. Taking advantages of competitive host-guest interaction and magnetic separation, highly efficient, reusable and simultaneous cholesterol detection and elimination can be achieved. The limit of detection is determined to be 4.3 nM, which is comparable or even lower than existing methods. The distinguished performance may attribute to the high loading efficiency and magnetic enrichment of nanoparticles. Besides, this efficient strategy is resistant to interfering substances, thus realizing sensitive cholesterol detection in real sample. Simultaneously, the bifunctional magnetic nanoparticles also have up to 95% cholesterol elimination efficiency, which is higher than previous reported methods. Furthermore, the nanoparticles are turned out to be reusable within 5 times without noticeable loss in cholesterol elimination efficiency. Therefore, the bifunctional magnetic nanoparticles fabricated here could hold great potential for simultaneous cholesterol detection and elimination in practical applications.
胆固醇是维持细胞内稳态和人体健康的必需化合物。敏感检测胆固醇和有效清除过量胆固醇已成为临床诊断和健康管理中的必要操作。迄今为止，同时进行胆固醇检测和消除任务仍然极具挑战性。在本研究中，设计并制备了双功能磁性纳米粒子（FeO@PDA-PBA-CD）来克服这一困难。利用竞争性主客体相互作用和磁分离的优势，可以实现高效、可重复使用和同时的胆固醇检测和消除。检测限确定为 4.3 nM，可与现有方法媲美，甚至更低。优异的性能可能归因于纳米粒子的高负载效率和磁性富集。此外，这种高效策略还能抵抗干扰物质，从而实现实际样品中灵敏的胆固醇检测。同时，双功能磁性纳米粒子对胆固醇的去除效率高达 95%，高于之前报道的方法。此外，这些纳米粒子在 5 次重复使用后仍保持较高的胆固醇去除效率，没有明显的损失。因此，所制备的双功能磁性纳米粒子在实际应用中具有同时进行胆固醇检测和消除的巨大潜力。
