Graduate School of Biotechnology, Kyung Hee University, Giheung-gu, Yongin-si, Gyeonggi-do, South Korea.
Tianjin Ginkingsen Health Technology Co., Ltd, Binhai Gaoxin, Tianjin, China.
J Cell Biochem. 2019 Jan;120(1):601-612. doi: 10.1002/jcb.27417. Epub 2018 Sep 8.
Excessive ultraviolet radiation usually causes skin photoaging, inflammation, and even photocarcinogenesis. UV radiation-generated reactive oxygen species (ROS) are a major contributing factor to photodamage. The flowers of Helianthus annuus L. have been reported to possess strong anti-inflammatory and antioxidant activity. However, there are few reports on the use of H. annuus L. to relieve UVB-induced photoaging.
In this study, we evaluated the protective effect of a 50% ethanol extract of H. annuus L. flower (HAF) against UVB-induced photodamage using normal human dermal fibroblasts.
The secretion of ROS, interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMPs), procollagen type I, and transforming growth factor-β1 (TGF-β1) was measured with kits. The messenger RNA levels of COX-2, iNOS, and TGF-α were measured by RT-PCR. The AP-1, MAPK, NFAT, and Nrf2 pathways were investigated by Western blot analysis.
HAF extract significantly blocked UVB-induced ROS and MMP (MMP-1 and MMP-3) production and procollagen type I reduction. Further study demonstrated that the photoaging inhibitory actions were related to promotion of Nrf2 nuclear translocation, upregulation of TGF-β1 level, and downregulation of AP-1 and MAPK phosphorylation. Importantly, HAF effectively inhibited UVB-induced VEGF and inflammatory cytokines such as IL-6, COX-2, iNOS, and TNF-α secretion, which might be involved in the regulation of the NFAT signaling pathway.
Our results indicate that HAF is a useful botanical source protecting against UVB-mediated skin photodamage.
过量的紫外线辐射通常会导致皮肤光老化、炎症，甚至光致癌。紫外线辐射产生的活性氧（ROS）是光损伤的主要因素。向日葵的花已被报道具有很强的抗炎和抗氧化活性。然而，关于利用向日葵来缓解 UVB 诱导的光老化的报道很少。
本研究采用正常的人皮肤成纤维细胞，评估向日葵花 50%乙醇提取物（HAF）对 UVB 诱导的光损伤的保护作用。
采用试剂盒测定 ROS、白细胞介素-6（IL-6）、血管内皮生长因子（VEGF）、基质金属蛋白酶（MMPs）、I 型前胶原和转化生长因子-β1（TGF-β1）的分泌。通过 RT-PCR 测定 COX-2、iNOS 和 TGF-α的信使 RNA 水平。通过 Western blot 分析研究 AP-1、MAPK、NFAT 和 Nrf2 通路。
HAF 提取物显著阻断了 UVB 诱导的 ROS 和 MMP（MMP-1 和 MMP-3）的产生以及 I 型前胶原的减少。进一步的研究表明，这种光老化抑制作用与促进 Nrf2 核易位、上调 TGF-β1 水平以及下调 AP-1 和 MAPK 磷酸化有关。重要的是，HAF 能有效抑制 UVB 诱导的 VEGF 和炎症细胞因子（如 IL-6、COX-2、iNOS 和 TNF-α）的分泌，这可能与 NFAT 信号通路的调节有关。
我们的结果表明，HAF 是一种有用的植物来源，可预防 UVB 介导的皮肤光损伤。
