Department of Orthopedic Surgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, 94720, USA.
J Biomed Mater Res B Appl Biomater. 2019 Apr;107(3):818-824. doi: 10.1002/jbm.b.34178. Epub 2018 Sep 8.
The repair and regeneration of loaded segmental bone defects is a challenge for both materials and biomedical science communities. Our recent work demonstrated the capability of bioactive glass in supporting bone healing and defect bridging using a rabbit femur segmental defect model without growth factors or bone marrow stromal cells (BMSCs). Here in the current work, a comprehensive in vitro evaluation of bioactive silicate (13-93) and borosilicate (2B6Sr) glass scaffolds was conducted to provide further understanding of their biological performances and to establish a correlation between in vitro and in vivo behaviors. Our in vitro evaluation using a murine MC3T3-E1 cell line confirmed the capability of both scaffolds to support cell attachment, vascular endothelial growth factor (VEGF) formation, and to stimulate mineral deposition and osteoblast marker gene expression. In particular, borosilicate (2B6Sr) glass showed a better capability in supporting the mineralization and gene expression than silicate (13-93) glass, consistent with a faster bone healing ability in vivo. The current in vitro results, combined with our previous in vivo findings, provide a strong basis for the further translational evaluation of bioactive glass scaffolds and for potential preclinical practice. © 2018 Wiley Periodicals, Inc. J. Biomed. Mater. Res. Part B, 2018. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 818-824, 2019.
负载性骨节段缺损的修复和再生是材料和生物医学科学界面临的挑战。我们最近的研究表明，生物活性玻璃在不使用生长因子或骨髓基质细胞（BMSCs）的情况下，通过兔股骨节段性缺损模型具有支持骨愈合和缺损桥接的能力。在目前的工作中，对生物活性硅酸盐（13-93）和硼硅酸盐（2B6Sr）玻璃支架进行了全面的体外评估，以进一步了解它们的生物学性能，并建立体外和体内行为之间的相关性。我们使用鼠 MC3T3-E1 细胞系进行的体外评估证实了两种支架都能够支持细胞附着、血管内皮生长因子（VEGF）的形成，并刺激矿物质沉积和成骨细胞标志物基因的表达。特别是，硼硅酸盐（2B6Sr）玻璃在支持矿化和基因表达方面的能力优于硅酸盐（13-93）玻璃，这与体内更快的骨愈合能力一致。目前的体外结果，结合我们之前的体内发现，为生物活性玻璃支架的进一步转化评估以及潜在的临床前实践提供了坚实的基础。©2018 年威利父子公司。J.生物医学材料研究。第 B 部分，2018 年。©2018 年威利父子公司。J 生物医学材料研究 B 部分：应用生物材料 107B:818-824,2019 年。
