Wang Xiaoyan, Chen Wei, Liu Qianqian, Gao Kai, Wang Gan, Gao Li, Liu Long
Department of Chemistry and Biology, College of Science, National University of Defense Technology, Changsha, Hunan, 410073, People's Republic of China.
Biomed Mater. 2017 Apr 28;12(2):025020. doi: 10.1088/1748-605X/aa65d8.
Mesoporous bioactive glass (MBG) has been demonstrated to play a vital role in bone tissue engineering due to its bioactivity, biocompatibility, and osteoinduction properties. Here, we report that MBG grafted with an amino group (MBG-NH) and MBG-NH adsorbed epidermal growth factor (EGF) (MBG-NH/EGF) sustained-release EGF, and MBG-NH2/EGF could accelerate osteoblast differentiation and mineralization in MC3T3-E1 cells. We found that MBG-NH could promote bone-like deposit formation and Ca deposition in vitro. Intriguingly, we observed that MBG-NH/EGF enhanced MC3T3-E1 cell adhesion. We also showed that extracellular signal-regulated kinase 1/2 (ERK1/2) was phosphorylated when MC3T3-E1 cells were cultured on MBG-NH/EGF. Interestingly, the transcription factor Runx2, important for osteoblast differentiation, was also activated when MC3T3-E1 cells were cultured on MBG-NH/EGF. We showed that MC3T3-E1 cells cultured on MBG-NH/EGF activating Runx2 was through ERK1/2 phosphorylation. Consistent with this survey, we observed that MC3T3-E1 cells cultured on MBG-NH/EGF accelerated osteoblastic marker gene expressions, including osteopontin (Opn) and osteocalcin (Ocn). Taken together, we conclude that the osteoblast differentiation and mineralization were accelerated in MC3T3-E1 cells cultured on MBG-NH/EGF through ERK-activated Runx2 pathway. These findings support the idea that MBG-NH/EGF is a potential biomaterial for bone tissue repair in bone defect-related diseases.
介孔生物活性玻璃（MBG）因其生物活性、生物相容性和骨诱导特性，已被证明在骨组织工程中发挥着至关重要的作用。在此，我们报告接枝有氨基的MBG（MBG-NH）以及吸附了表皮生长因子（EGF）的MBG-NH（MBG-NH/EGF）能够持续释放EGF，并且MBG-NH2/EGF可以加速MC3T3-E1细胞中的成骨细胞分化和矿化。我们发现MBG-NH能够在体外促进类骨沉积物形成和钙沉积。有趣的是，我们观察到MBG-NH/EGF增强了MC3T3-E1细胞的黏附。我们还表明，当MC3T3-E1细胞在MBG-NH/EGF上培养时，细胞外信号调节激酶1/2（ERK1/2）会发生磷酸化。有趣的是，当MC3T3-E1细胞在MBG-NH/EGF上培养时，对成骨细胞分化很重要的转录因子Runx2也被激活。我们表明，在MBG-NH/EGF上培养的MC3T3-E1细胞激活Runx2是通过ERK1/2磷酸化实现的。与该研究结果一致，我们观察到在MBG-NH/EGF上培养的MC3T3-E1细胞加速了成骨细胞标志物基因的表达，包括骨桥蛋白（Opn）和骨钙素（Ocn）。综上所述，我们得出结论，在MBG-NH/EGF上培养的MC3T3-E1细胞中，通过ERK激活的Runx2途径加速了成骨细胞分化和矿化。这些发现支持了MBG-NH/EGF是骨缺损相关疾病中骨组织修复的潜在生物材料这一观点。
