Esumi M, Aritaka T, Arii M, Suzuki K, Tanikawa K, Mizuo H, Mima T, Shikata T
Cancer Res. 1986 Nov;46(11):5767-71.
The hepatitis B virus genome is integrated in cellular DNA of human hepatocellular carcinoma from hepatitis B surface antigen-positive patients. Using this phenomenon, we determined the clonal origin of hepatocellular carcinoma from the integration mode of hepatitis B virus DNA. The molecular size and the number of restriction fragments of integrated hepatitis B virus DNA in several parts of tumors in the same liver and in metastatic tumors were compared by Southern blot analysis. Of 14 cases of hepatoma, 13 cases were monoclonal; in one case, a different clone of hepatoma was found in one part of the tumor. In three of 13 cases of monoclonal hepatoma, metastatic tumors in lymph nodes and the lung were also examined and found to be the same clone as the liver tumors. These results indicate that hepatocellular carcinomas were usually generated from a single tumor cell even though tumor cells spread in the liver and invaded other organs for a long time. Development of different clones of tumor was apparently unusual but was observed in one case of hepatocellular carcinoma.
乙肝表面抗原阳性患者的人肝细胞癌的细胞DNA中整合有乙肝病毒基因组。利用这一现象，我们从乙肝病毒DNA的整合模式确定了肝细胞癌的克隆起源。通过Southern印迹分析比较了同一肝脏肿瘤多个部位以及转移瘤中整合的乙肝病毒DNA的分子大小和限制性片段数量。在14例肝癌病例中，13例为单克隆；在1例中，肿瘤的一个部位发现了不同的肝癌克隆。在13例单克隆肝癌中的3例中，还检查了淋巴结和肺部的转移瘤，发现与肝脏肿瘤为同一克隆。这些结果表明，即使肿瘤细胞在肝脏中扩散并长期侵犯其他器官，肝细胞癌通常也由单个肿瘤细胞产生。肿瘤不同克隆的发生显然不常见，但在1例肝细胞癌中观察到了这种情况。
