Phillips E R, Ruch R J
Cancer Res. 1986 Nov;46(11):5864-8.
Five of six rat sarcomas, induced by the Schmidt-Ruppin (SR) strain of avian tumor virus, expressed a Mr 60,000 tumor cell surface antigen (TSA), immunoprecipitable from non-ionic detergent extracts. Expression of the antigen was exclusive to rat cells transformed by the SR virus strain. Moreover, expression of TSA appeared restricted by cell type. The five TSA-positive SR-transformed rat cell lines tested were apparently of fibroblastic origin, but cultured rat cerebral endothelial cells (RCE-T1), transformed by SR virus, showed no expression of TSA. However, the antigen emerged on cultured tumors obtained after histoincompatible transplantation of these cells into newborn rats of another strain (tumor digest cells). Investigation of TSA for immunological relationship to viral structural antigens and the src gene product indicated that the TSA is distinct from any of these and more probably derives from a virus-directed alteration in a host molecule.
由禽肿瘤病毒施密特 - 鲁平（SR）株诱导产生的6个大鼠肉瘤中，有5个表达了一种分子量为60,000的肿瘤细胞表面抗原（TSA），该抗原可从非离子去污剂提取物中免疫沉淀出来。该抗原的表达仅限于被SR病毒株转化的大鼠细胞。此外，TSA的表达似乎受细胞类型限制。所检测的5个TSA阳性的SR转化大鼠细胞系显然起源于成纤维细胞，但经SR病毒转化的培养大鼠脑内皮细胞（RCE - T1）未显示TSA表达。然而，将这些细胞组织不相容移植到另一品系新生大鼠后获得的培养肿瘤（肿瘤消化细胞）中出现了该抗原。对TSA与病毒结构抗原及src基因产物的免疫关系研究表明，TSA与这些抗原均不同，更可能源自宿主分子中病毒导向的改变。
