Institute for Geriatrics and Rehabilitation, Beijing Geriatric Hospital, Beijing University of Chinese Medicine, Beijing 100095, China.; Department of Laboratory Center, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.
Institute for Geriatrics and Rehabilitation, Beijing Geriatric Hospital, Beijing University of Chinese Medicine, Beijing 100095, China.; Department of Bacteriology and Immunology, Beijing Key Laboratory on Drug-Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute/Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
Clin Chim Acta. 2018 Dec;487:369-376. doi: 10.1016/j.cca.2018.09.008. Epub 2018 Sep 6.
The current diagnostic methods for tuberculosis (TB) have several limitations. Although commercial serological tests based on antibody detection are available, their variable accuracies limit their roles in the clinic. The aim of this study was to discover the improved biomarkers for TB disease by investigating the serum profiles of IgG and IgM antibodies against nearly all Mycobacterium tuberculosis (MTB) antigens in 36 active TB patients and 18 healthy controls (HCs) using proteome microarrays. Our results revealed that multiple antigens could induce stronger serum IgG or IgM responses in TB patients compared to HCs, among them, Rv2026c and Rv2421c were further validated by ELISA with sera from 221 samples and showed the moderate performance in diagnosing TB by receiver operating characteristic analysis. Moreover, logistic regression analysis was performed to establish a combined panel that provided better sensitivity and specificity at 82.5% and 88.12%, respectively, than single antigens in the diagnosis of active TB. Furthermore, the antibody reactivity against Rv2026c and Rv2421c was correlated with clinical backgrounds. These results suggest that the combination of different antigens and classes of antibodies could provide promise and encouragement in developing an efficient serological test for the diagnosis of active TB.
目前用于诊断结核病（TB）的方法存在一些局限性。尽管已经有基于抗体检测的商业化血清学检测方法，但它们的准确性各不相同，限制了它们在临床中的应用。本研究旨在通过使用蛋白质组微阵列技术，研究针对几乎所有结核分枝杆菌（MTB）抗原的 IgG 和 IgM 抗体的血清谱，发现用于 TB 疾病的改良生物标志物。我们的研究结果表明，与健康对照组（HCs）相比，多种抗原可诱导 TB 患者产生更强的血清 IgG 或 IgM 反应，其中 Rv2026c 和 Rv2421c 进一步通过 ELISA 进行了验证，结果显示在 221 个样本血清中具有中等的 TB 诊断性能，ROC 分析表明具有较高的诊断效能。此外，我们还进行了逻辑回归分析，建立了一个联合检测面板，与单一抗原相比，该联合检测面板在诊断活动性 TB 时具有更好的敏感性（82.5%）和特异性（88.12%）。此外，针对 Rv2026c 和 Rv2421c 的抗体反应与临床背景有关。这些结果表明，不同抗原和抗体类别组合可能为开发用于诊断活动性 TB 的高效血清学检测方法提供希望和鼓励。
