Institute of Cytology and Genetics of Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia.
Institute of Cytology and Genetics of Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia.
Mol Phylogenet Evol. 2018 Dec;129:280-290. doi: 10.1016/j.ympev.2018.09.002. Epub 2018 Sep 6.
Organellar genomes may shed light on complicated patterns of plant evolution at inter- and intraspecies level. Primary structure of plastid genomes sequenced in this study and taken from public databases was characterised and compared in 22 diverse, mostly wild representatives of the genus Pisum (peas). Phylogenetic trees reconstructed via Bayesian approach on the basis of entire plastid genomes resembled those reconstructed on the basis of a nuclear gene His5 coding for a minor histone H1 subtype. They reveal Pisum fulvum as an early divergence of the genus but do not support other taxonomical subdivisions. The positions of three accessions, classified as P. sativum subsp. elatius (the wild subspecies of the common pea), appeared quite unexpected. On the entire plastid genome tree, two accessions, from the Black Sea area of Turkey and Georgia, clustered with representatives of another species, P. fulvum, while the other, from Greece, was the first divergence of the P. sativum branch. We suppose these unusual plastid genomes to be ancient lineages ascending to a 'missing link' between P. fulvum and P. sativum, represented by accession Pe 013 from Turkey. Accessions with common pea appearance but deeply diverged plastids could occur through occasional crossing of diverged pea lines in the past and biparental plastid inheritance, both events being possible in peas.
细胞器基因组可能揭示物种间和种内植物进化的复杂模式。本研究从公共数据库中测序并选取了 22 种不同的、主要是野生豌豆属（豌豆）代表，对其质体基因组的一级结构进行了特征描述和比较。基于整个质体基因组构建的贝叶斯系统发育树与基于核基因 His5 构建的系统发育树相似，His5 编码一个次要组蛋白 H1 亚型。它们表明，Pisum fulvum 是该属的早期分支，但不支持其他分类学划分。三个豌豆属的分类为 P. sativum subsp. elatius（普通豌豆的野生亚种）的 accession 的位置出乎意料。在整个质体基因组树上，来自土耳其和格鲁吉亚黑海地区的两个 accession 与 P. fulvum 的代表聚类，而来自希腊的另一个 accession 是 P. sativum 分支的第一个分支。我们假设这些不寻常的质体基因组是古老的谱系，它们与 P. fulvum 和 P. sativum 之间的“缺失环节”有关，代表是来自土耳其的 accession Pe 013。具有普通豌豆外观但质体深度分化的 accession 可能是由于过去不同豌豆谱系的偶然杂交和双亲质体遗传，这两种事件在豌豆中都是可能发生的。
