Stavridou Evangelia, Lagiotis Georgios, Karapetsi Lefkothea, Osathanunkul Maslin, Madesis Panagiotis
Institute of Applied Biosciences, Centre for Research and Technology, Thermi, GR-570 01 Thessaloniki, Greece.
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Plants (Basel). 2020 Apr 9;9(4):479. doi: 10.3390/plants9040479.
Pea ( L.), one of the most important legume crops worldwide, has been traditionally cultivated in Lesser Cyclades since ancient times. The commonly known traditional pea cultivar, 'Katsouni', is endemic to the islands of Amorgos and Schinoussa and is of great local economic importance. Despite the widespread cultivation of 'Katsouni' in both islands, it is still unknown whether the current Schinoussa and Amorgos pea populations are distinct landraces, and if they have common evolutionary origin. To assist conservation and breeding of the pea crop, the genetic diversity and phylogenetic relationships of 39 pea samples from Amorgos and 86 from Schinoussa were studied using DNA barcoding and ISSR marker analyses. The results indicate that both populations are different landraces with distinct geographical distribution and are more closely related to subsp than the and species. Further characterization of the 'Katsouni' landraces for functional polymorphisms regarding pathogen resistance, revealed susceptibility to the powdery mildew ( DC.). This work represents the first investigation on the genetic diversity and population structure of the 'Katsouni' cultivar. Exploiting the local genetic diversity of traditional landraces is fundamental for conservation practices and crop improvement through breeding strategies.
豌豆（L.）是全球最重要的豆类作物之一，自古以来就在小基克拉泽斯群岛传统种植。广为人知的传统豌豆品种“卡苏尼”是阿莫尔戈斯岛和希努萨岛特有的品种，具有重要的地方经济意义。尽管“卡苏尼”在这两个岛屿上广泛种植，但目前希努萨岛和阿莫尔戈斯岛的豌豆种群是否为不同的地方品种，以及它们是否有共同的进化起源仍不清楚。为了协助豌豆作物的保护和育种，利用DNA条形码和ISSR标记分析研究了来自阿莫尔戈斯岛的39个豌豆样本和来自希努萨岛的86个豌豆样本的遗传多样性和系统发育关系。结果表明，这两个种群是具有不同地理分布的不同地方品种，与subsp的关系比与和物种的关系更密切。对“卡苏尼”地方品种在病原菌抗性功能多态性方面的进一步表征，揭示了其对白粉病（DC.）的易感性。这项工作是对“卡苏尼”品种遗传多样性和种群结构的首次研究。利用传统地方品种的当地遗传多样性对于通过育种策略进行保护实践和作物改良至关重要。
