Sureau C, Romet-Lemonne J L, Mullins J I, Essex M
Cell. 1986 Oct 10;47(1):37-47. doi: 10.1016/0092-8674(86)90364-8.
Closed-circular HBV DNA was introduced into cells of the established human hepatoma culture HepG2. The culture medium of one of 40 single-cell clones contained HBV surface antigen (HBsAg), core-related antigens (HBc/eAg), and HBV DNA sequences. HBV DNA and DNA polymerase activity were detected in particles resembling both nucleocapsids and complete virions (Dane particles). Intracellular integrated and extrachromosomal HBV DNA sequences were detected. Relaxed-circular and single-stranded forms of viral DNA were identified as likely replicative intermediates of the HBV genome. In conclusion, in vitro production of Dane-like particles by transformed human hepatocytes has been achieved. This model should be valuable as a cell culture system for studying virus replication and virus-host cell interactions.
将闭环乙肝病毒（HBV）DNA导入已建立的人肝癌细胞系HepG2细胞中。40个单细胞克隆中的一个克隆的培养基中含有乙肝病毒表面抗原（HBsAg）、核心相关抗原（HBc/eAg）和HBV DNA序列。在类似核衣壳和完整病毒体（Dane颗粒）的颗粒中检测到HBV DNA和DNA聚合酶活性。检测到细胞内整合的和染色体外的HBV DNA序列。病毒DNA的松弛环形式和单链形式被鉴定为可能是HBV基因组的复制中间体。总之，已实现了转化的人肝细胞在体外产生类Dane颗粒。该模型作为研究病毒复制和病毒-宿主细胞相互作用的细胞培养系统应具有重要价值。
