Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Taiwan, ROC.
Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Taiwan, ROC; Department of Pediatrics, Kaohsiung Municipal Hsiao-Kang Hospital, Taiwan, ROC.
J Microbiol Immunol Infect. 2018 Dec;51(6):829-838. doi: 10.1016/j.jmii.2018.08.006. Epub 2018 Aug 27.
BACKGROUND/PURPOSE: Dengue disease is widespread in tropical and sub-tropical regions. Severe dengue infection is characterized by plasma leakage, fluid accumulation, severe bleeding, or vital organ impairment. Bleeding is a critical complication of dengue disease. However, the biomarkers of dengue disease are still unknown. Macrophages have a distinct polarization phenotype related to M1/M2 classification. Macrophage polarization toward the pro-inflammatory M1 phenotype is considered critical for efficient antiviral immune responses, whereas the anti-inflammatory M2 phenotype is considered essential for tissue remodeling. We investigated macrophage polarization patterns in the peripheral blood of pediatric patients with dengue disease.
Medical records and laboratory data were collected from 23 pediatric healthy controls and 100 dengue disease samples from 50 dengue patients. Macrophage polarization-related surface markers were assessed using flow cytometry.
The percentage of macrophages in the peripheral blood was higher in dengue patients than in the healthy controls. The percentages of M2a and M2c macrophage subsets were higher and the percentage of M1 macrophage subset was lower in dengue patients than in healthy controls. However, the percentages of M1, M2a and M2b macrophage subsets in dengue patients with bleeding tendency were lower than that without bleeding tendency. The percentages of M2a, M2b, and M2c macrophage subsets were positively correlated with platelet counts.
Decreased the percentages of M2 macrophage subsets in pediatric dengue patients are associated with bleeding tendency and lower platelet counts.
背景/目的：登革热疾病广泛流行于热带和亚热带地区。重症登革热感染的特征为血浆渗漏、体液蓄积、严重出血或重要器官损伤。出血是登革热疾病的一种严重并发症。然而，登革热疾病的生物标志物仍不清楚。巨噬细胞具有独特的极化表型，与 M1/M2 分类相关。巨噬细胞向促炎 M1 表型极化被认为对有效的抗病毒免疫反应至关重要，而抗炎 M2 表型被认为对组织重塑至关重要。我们研究了登革热患儿外周血中巨噬细胞的极化模式。
收集了 23 名儿科健康对照者和 50 例登革热患者的 100 份登革热疾病样本的病历和实验室数据。采用流式细胞术评估巨噬细胞极化相关的表面标志物。
登革热患者外周血中的巨噬细胞百分比高于健康对照组。登革热患者中 M2a 和 M2c 巨噬细胞亚群的百分比较高，而 M1 巨噬细胞亚群的百分比较低。然而，有出血倾向的登革热患者中 M1、M2a 和 M2b 巨噬细胞亚群的百分比低于无出血倾向的患者。M2a、M2b 和 M2c 巨噬细胞亚群的百分比与血小板计数呈正相关。
儿科登革热患者中 M2 巨噬细胞亚群的百分比降低与出血倾向和血小板计数降低有关。
