Department of Immunology and genetic, Urmia University of Medical Sciences, Urmia, Iran.
Department of Genetics, Urmia University of Medical Sciences, Urmia, Iran.
J Neuroimmunol. 2018 Oct 15;323:105-108. doi: 10.1016/j.jneuroim.2018.08.004. Epub 2018 Aug 8.
Multiple sclerosis (MS), is an autoimmune disorder of central nervous system (CNS) characterized by inflammation and demyelination. Self-tolerance impairment is considered to be induced by a combination of inherited susceptibility and environmental agents. In this work, we demonstrate that a reduction in the comparative expression of well-known inhibitory receptors (i.e., CTLA-4, PD-1, and TIM-3) is importantly linked with MS patients compared to healthy controls. The relative expression of interested genes was performed on peripheral blood mononuclear cells (PBMCs), by using quantitative real time-PCR (qRT-PCR). Our data highlighted the role of inhibitory receptors in the maintenance of immune homeostasis in autoimmune disease.
多发性硬化症（MS）是一种中枢神经系统（CNS）自身免疫性疾病，其特征为炎症和脱髓鞘。自身耐受的损害被认为是由遗传易感性和环境因素的组合诱导的。在这项工作中，我们证明了与健康对照组相比，多发性硬化症患者中一些众所周知的抑制性受体（即 CTLA-4、PD-1 和 TIM-3）的相对表达显著降低。通过实时定量 PCR（qRT-PCR）在外周血单核细胞（PBMC）中检测感兴趣基因的相对表达。我们的数据强调了抑制性受体在自身免疫性疾病中维持免疫稳态中的作用。
