聚肌胞诱导免疫反应的变异性：对临床前母体免疫激活模型的影响。
Variability in PolyIC induced immune response: Implications for preclinical maternal immune activation models.
机构信息
Department of Psychiatry and Behavioral Sciences, University of California, Davis, USA; The MIND Institute, University of California, Davis, USA.
Department of Public Health Sciences, University of California, Davis, USA.
出版信息
J Neuroimmunol. 2018 Oct 15;323:87-93. doi: 10.1016/j.jneuroim.2018.06.014. Epub 2018 Jun 28.
Abstract
Maternal infection during pregnancy may increase the risk of offspring neurodevelopmental disorders. The preclinical Polyinosinic-polycytidylic acid (PolyIC) model has become one of the most widely used approaches in maternal immune activation (MIA) research. However, variability in molecular weight may impact the immune activating potential of PolyIC. Nulliparous rats injected with high molecular weight PolyIC exhibit pronounced cytokine response and sickness behavior that was not observed in rats injected low molecular weight PolyIC. Although an essential next step is to extend these studies to pregnant animals, the preliminary results suggest that PolyIC molecular weight is an important experimental design consideration.
摘要
母体怀孕期间感染可能会增加后代神经发育障碍的风险。聚肌胞苷酸（PolyIC）的临床前模型已成为母体免疫激活（MIA）研究中最广泛使用的方法之一。然而，分子量的变化可能会影响 PolyIC 的免疫激活潜力。注射高分子量 PolyIC 的未产大鼠表现出明显的细胞因子反应和疾病行为，而注射低分子量 PolyIC 的大鼠则没有观察到这种反应。尽管下一步的重要步骤是将这些研究扩展到怀孕动物，但初步结果表明 PolyIC 分子量是一个重要的实验设计考虑因素。
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