Rashed Reham, Shafik Roxan E, Shafik Nevine F, Shafik Hanan E
Department of Clinical Pathology, National Cancer Institute, Cairo University, Cairo, Egypt.
Department of Medical Oncology, National Cancer Institute, Cairo University, Cairo, Egypt.
J Cancer Res Ther. 2018 Jul-Sep;14(5):1083-1086. doi: 10.4103/0973-1482.187367.
Acute myeloid leukemia (AML) is a cytogenetically and molecularly heterogeneous diseases, and characterization of transforming genetic events is becoming increasingly important. Interleukins (ILs) are a diverse set of small cell signaling protein molecules. Single nucleotide polymorphisms (SNPs) of ILs alter their function, increasing susceptibility to different diseases.
We investigated the association between polymorphism in IL-10 -819T/C (rs1800871) and the risk of AML in the Egyptian population. DNA was isolated from bone marrow of 80 newly diagnosed adult AML patients, and 85 age- and sex-matched controls. Genetic analysis of IL-10 SNPs at -819T/C was assayed by polymerase chain reaction-restriction fragment length polymorphism.
Genetic analysis of IL-10 revealed that the Egyptians have high -819T allele frequencies in apparently healthy controls, whereas -819CC genotype and the -819C allele frequencies in the AML group were higher than in the controls (P = 0.000086). The study suggested that subjects carrying the rs1800871CC genotype and C allele had a significantly increased risk for AML.
IL-10 SNP at -819 was associated with enhanced AML risk, suggesting that rs1800871 provides clue for future studies and early detection of AML.
急性髓系白血病（AML）是一种细胞遗传学和分子水平上的异质性疾病，确定转化遗传事件的特征变得越来越重要。白细胞介素（ILs）是一组多样的小细胞信号蛋白分子。ILs的单核苷酸多态性（SNPs）会改变其功能，增加对不同疾病的易感性。
我们研究了埃及人群中IL-10 -819T/C（rs1800871）多态性与AML风险之间的关联。从80例新诊断的成年AML患者以及85例年龄和性别匹配的对照者的骨髓中分离DNA。通过聚合酶链反应-限制性片段长度多态性分析IL-10在-819T/C处的SNPs。
IL-10的基因分析显示，在明显健康的对照者中，埃及人具有较高的-819T等位基因频率，而AML组中的-819CC基因型和-819C等位基因频率高于对照组（P = 0.000086）。该研究表明，携带rs1800871CC基因型和C等位基因的受试者患AML的风险显著增加。
IL-10在-819处的SNP与AML风险增加相关，表明rs1800871为未来AML的研究和早期检测提供了线索。
