Yao Chen-Jiao, Du Wei, Chen Hai-Bing, Xiao Sheng, Wang Cheng-Hong, Fan Zi-Li
Department of Hematology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.
Asian Pac J Cancer Prev. 2013;14(4):2439-42. doi: 10.7314/apjcp.2013.14.4.2439.
We investigated the possible association of interleukin-10 (IL-10) single nucleotide polymorphisms (SNPs) and susceptibility to acute myeloid leukemia (AML) in 115 patients and 137 healthy controls. Genetic analysis of IL-10 SNPs at -819 and -592 was carried out with the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) approach. The IL-10 mRNA expression of AML patients and controls with different genotype was detected by real-time quantitative polymerase chain reaction (RT-PCR). Genetic analysis of IL-10 revealed that the -819AA genotype frequencies and the -819A allele frequencies in the AML group were higher than in the controls (59.1% vs 40.9%; 75.6% vs 63.9%, respectively); there were remarkable differences in -819T/C and -592A/C gene distribution (P<0.05) and the TA haploid frequencies were higher in the AML group (75.6% vs 63.9%, P<0.05). IL-10 mRNA expression in incipient AML patients was obvious higher than the non- tumor group and the remission group (7.78?10-3 vs 2.43?10-3, 3.64?10-3, P<0.05).The study suggested that the haploid TA and genotype TA/TA may be associated with AML in Han people in Hunan province.The IL-10 SNPs at -819 and -592 sites were associated with AML and may affect IL-10 mRNA expression in AML patients.
我们在115例急性髓系白血病（AML）患者和137名健康对照者中，研究了白细胞介素-10（IL-10）单核苷酸多态性（SNP）与急性髓系白血病易感性之间的可能关联。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对-819和-592位点的IL-10 SNP进行基因分析。通过实时定量聚合酶链反应（RT-PCR）检测不同基因型AML患者和对照者的IL-10 mRNA表达。IL-10基因分析显示，AML组中-819AA基因型频率和-819A等位基因频率高于对照组（分别为59.1%对40.9%；75.6%对63.9%）；-819T/C和-592A/C基因分布存在显著差异（P<0.05），AML组中TA单倍型频率更高（75.6%对63.9%，P<0.05）。初发AML患者的IL-10 mRNA表达明显高于非肿瘤组和缓解组（7.78×10-3对2.43×10-3、3.64×10-3，P<0.05）。该研究提示，单倍型TA和基因型TA/TA可能与湖南省汉族人群的AML有关。-819和-592位点的IL-10 SNP与AML有关，可能影响AML患者的IL-10 mRNA表达。
