Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Biotechnology and Bioscience Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Andrology. 2020 Sep;8(5):1167-1173. doi: 10.1111/andr.12801. Epub 2020 Jun 5.
Recent evidence suggests that routine semen analysis has limited power to predict male fertility. Several efforts have been made to best discriminate between fertile and subfertile men using several sperm features. So, there is a need to more reliable assays for providing molecular data to evaluate sperm quality and predict male fertility.
Present study aimed to investigate relationship between sperm miR-26a-5p and its target PTEN transcript content and sperm parameters in unexplained infertile and healthy fertile men.
Sperm parameters and transcript content of miR-26a-5p and PTEN in ejaculated spermatozoa were assessed in fertile (n = 15) and normozoospermic infertile (n = 15) men.
Sperm transcript levels of miR-26a-5p were lower in unexplained infertile men than in fertile controls (P = .021). High sperm PTEN expression is correlated with low miR-26a-5p transcript levels in ejaculated spermatozoa. High levels of sperm miR-26a-5p transcript were associated with high sperm motility and normal morphology. Receiver operating characteristic curve (ROC) analysis indicated miR-26a-5p has a fairly good diagnostic value to distinguish fertile and unexplained infertile men with an area under curve (AUC) of 0.747 (95% confidence interval: 0.560-0.933; P = .021).
This study suggests that sperm miR-26a-5p and its target PTEN transcript content may contribute to infertility etiology in unexplained infertile patients.
miR-26a-5p has a potential to use as a diagnostic biomarker and provide new therapeutic approaches for male infertility.
最近的证据表明，常规精液分析预测男性生育能力的能力有限。已经做出了一些努力，使用几种精子特征来最好地区分有生育能力和生育能力低下的男性。因此，需要更可靠的检测方法来提供分子数据，以评估精子质量并预测男性生育能力。
本研究旨在探讨不明原因不育和健康有生育能力男性的精子 miR-26a-5p 及其靶标 PTEN 转录本含量与精子参数之间的关系。
评估了有生育能力（n=15）和正常精子不育（n=15）男性的精子参数和 miR-26a-5p 和 PTEN 的转录本含量。
不明原因不育男性的精子 miR-26a-5p 转录本水平低于有生育能力的对照组（P=0.021）。高精子 PTEN 表达与射出精子中低 miR-26a-5p 转录本水平相关。高精子 miR-26a-5p 转录本水平与高精子活力和正常形态相关。受试者工作特征曲线（ROC）分析表明，miR-26a-5p 具有区分有生育能力和不明原因不育男性的相当好的诊断价值，曲线下面积（AUC）为 0.747（95%置信区间：0.560-0.933；P=0.021）。
本研究表明，精子 miR-26a-5p 及其靶标 PTEN 转录本含量可能导致不明原因不育患者的不育病因。
miR-26a-5p 具有作为诊断生物标志物的潜力，并为男性不育提供新的治疗方法。
