Jacob C O, Vaerman J P
Immunology. 1986 Sep;59(1):129-33.
There is accumulating evidence concerning the possible importance of secretory IgA antibodies in defence mechanisms against infections of the gastrointestinal tract, including cholera. Intestinal IgA antibodies are also thought to play a major role in protection against the diarrhoeogenic effects of cholera toxin. We therefore attempted to induce secretory IgA antibodies towards a reactive synthetic peptide from the cholera toxin B subunit sequence. We report that rat biliary secretory IgA antibodies against the CTP3 peptide (residues 50-64 of the B subunit) were obtained by three intra-Peyer's patch immunizations, at 2-week intervals, with CTP3 conjugated to tetanus toxoid in complete Freund's adjuvant. Purified secretory IgA fractions from bile of such immunized rats reacted with the carrier toxoid, but also with the CTP3 peptide, and with the native cholera toxin, they also partially neutralized its biological activity, as assayed by inhibition of in vitro cholera toxin-induced cAMP production in mouse thymocytes.
越来越多的证据表明，分泌型IgA抗体在包括霍乱在内的胃肠道感染防御机制中可能具有重要作用。肠道IgA抗体也被认为在抵御霍乱毒素的致腹泻作用中起主要作用。因此，我们试图诱导针对霍乱毒素B亚基序列的反应性合成肽产生分泌型IgA抗体。我们报告，通过每隔2周在派伊尔氏结内进行三次免疫接种，将CTP3与破伤风类毒素在完全弗氏佐剂中偶联，获得了针对CTP3肽（B亚基第50 - 64位氨基酸残基）的大鼠胆汁分泌型IgA抗体。从这些免疫大鼠胆汁中纯化的分泌型IgA组分不仅与载体类毒素反应，还与CTP3肽以及天然霍乱毒素反应，并且它们还部分中和了霍乱毒素的生物学活性，这是通过抑制小鼠胸腺细胞中体外霍乱毒素诱导的cAMP产生来测定的。
