Wasik M, Milgrom F
Immunol Invest. 1986 Jun;15(4):333-8. doi: 10.3109/08820138609052952.
Two cell lines secreting Paul-Bunnell antibodies were established by means of Epstein-Barr virus-induced immortalization of B lymphocytes from patients with infectious mononucleosis. Progressive loss of antibody production, most probably due to a shutdown of antibody secretion by individual cells was, however, observed. The first of the established cell lines lost its secretion ability after 10 weeks, and the second after 33 weeks of culture. Further improvements of the technique are necessary to stabilize the antibody secretion.
通过爱泼斯坦 - 巴尔病毒诱导传染性单核细胞增多症患者的B淋巴细胞永生化，建立了两种分泌保罗 - 邦内尔抗体的细胞系。然而，观察到抗体产生逐渐丧失，这很可能是由于单个细胞的抗体分泌停止所致。第一个建立的细胞系在培养10周后失去了分泌能力，第二个在培养33周后失去了分泌能力。为了稳定抗体分泌，有必要进一步改进该技术。
