Andrews Daniel D T, Franz-Odendaal Tamara A
Department of Biology, Mount Saint Vincent University.
Department of Biology, Mount Saint Vincent University;
J Vis Exp. 2018 Aug 25(138):57619. doi: 10.3791/57619.
The embryonic chicken is commonly used as a reliable model organism for vertebrate development. Its accessibility and short incubation period makes it ideal for experimentation. Currently, the study of these developmental pathways in the chicken embryo is conducted by applying inhibitors and drugs at localized sites and at low concentrations using a variety of methods. In vitro tissue culturing is a technique that enables the study of tissues separated from the host organism, while simultaneously bypassing many of the physical limitations present when working with whole embryos, such as the susceptibility of embryos to high doses of potentially lethal chemicals. Here, we present an organotypic culturing protocol for culturing the embryonic chicken half head in vitro, which presents new opportunities for the examination of developmental processes beyond the currently established methods.
胚胎鸡通常被用作研究脊椎动物发育的可靠模式生物。它易于获取且孵化期短，使其成为实验的理想选择。目前，通过使用多种方法将抑制剂和药物以低浓度应用于鸡胚的局部位点来开展对这些发育途径的研究。体外组织培养是一种能够研究从宿主生物体分离出来的组织的技术，同时还能规避在处理完整胚胎时存在的许多物理限制，比如胚胎对高剂量潜在致死化学物质的易感性。在此，我们展示一种用于体外培养胚胎鸡半头的器官型培养方案，该方案为超越现有方法研究发育过程提供了新机会。
