Arancia G, Ragona G, Rocchi G, Donelli G
Int J Cancer. 1986 Oct 15;38(4):549-52. doi: 10.1002/ijc.2910380415.
Ultrastructural modifications are described in the plasma membrane of in vitro established human B cells. By the freeze-fracture technique, intramembrane particles (IMPs) are quantified in B lymphocytes following Epstein-Barr virus (EBV) transformation in vitro, and in B-lymphoma (Burkitt-type) cells, either positive or negative for EBV genome. Analysis shows an overall increase in IMP density as compared to normal controls. Differences are observed between the protoplasmic and exoplasmic faces of fractured membranes as well as among in vitro transformed and clearly neoplastic cells. Results indicate that conformational changes in IMP distribution parallel neoplastic evolution of transformed cells.
本文描述了体外培养的人B细胞质膜的超微结构变化。通过冷冻蚀刻技术，对体外经爱泼斯坦-巴尔病毒（EBV）转化的B淋巴细胞以及EBV基因组呈阳性或阴性的B淋巴瘤（伯基特型）细胞中的膜内颗粒（IMP）进行定量分析。分析表明，与正常对照相比，IMP密度总体增加。在断裂膜的原生质面和外质面之间以及体外转化细胞和明显的肿瘤细胞之间观察到差异。结果表明，IMP分布的构象变化与转化细胞的肿瘤进化平行。
